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Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR

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Diagnostic Virology Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 12))

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Abstract

Human caliciviruses (HuCVs) and astroviruses are single-stranded RNA viruses that cause acute gastroenteritis in humans. HuCVs include several prototypes of small, round-structured viruses (SRSVs) as well as morphologically typical caliciviruses. Recent genetic characterization of HuCVs has divided them into three genogroups: Norwalk virus (NV), Snow Mountain agent (SMA), and Sapporo (Sapp) viruses (13). Astroviruses include at least seven antigenic types (4). Complete genomic sequences of astrovirus types 1 and 2 and partial sequences of other strains have been reported (57). In both families, differences in antigenic types appear to correspond to differences in genetic groups.

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© 1998 Humana Press Inc.

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Jiang, X., Matson, D.O. (1998). Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR. In: Stephenson, J.R., Warnes, A. (eds) Diagnostic Virology Protocols. Methods in Molecular Medicine™, vol 12. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-479-8:19

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  • DOI: https://doi.org/10.1385/0-89603-479-8:19

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-479-2

  • Online ISBN: 978-1-59259-596-9

  • eBook Packages: Springer Protocols

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