Abstract
cDNA cloning from a library is now a routine laboratory practice. Hybridization screening with either radiolabeled or nonradiolabeled probes, which is laborious and time-consuming, has been commonly used. Application of the polymerase chain reaction (PCR) is surprisingly expanded (1–3). Here a new application for cDNA library screening is reported: rapid cloning of full-length cDNAs by screening pools of cDNAs by PCR (RC-PCR) (4). This PCR-based cDNA screening technique is applicable to both bacteria and phage libraries.
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References
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© 1997 Humana Press Inc.
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Takumi, T. (1997). Use of PCR for cDNA Library Screening. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:339
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DOI: https://doi.org/10.1385/0-89603-483-6:339
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59259-553-2
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