Abstract
Evanescent wave immunobiosensors use antibodies immobilized at the surface of a waveguide to form a sensing device. Fluorescence-based evanescent wave biosensors form a fluorescent complex at the surface of the waveguide when the antigen of interest is present (e.g., a sandwich assay or direct binding of a fluorescent analyte) or alternatively, the nonfluorescent analyte may compete with a fluorescent analog of the analyte for binding, as in a competitive immunoassay format. Light propagating through the waveguide extends a short distance into the surrounding medium and excites the immobilized fluorophore. The waveguide also collects the emitted fluorescence and carries this light to a photodetector.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Golden, J. P., Anderson, G. P., Ogert, R. A., Breslm, K. A., and Ligler, F. S. (1992) An evanescent wave fiber optic biosensor: challenges for real world sensing. SPIE Proc 1796, 2–8.
Thompson, R. B. (1991) Fluorescence-based fiber-optic sensors, in Topics in Fluorescence Spectroscopy, vol 2 Principles (Lakowicz, J. R., ed.), Plenum, New York, pp. 345–365.
Thompson, R. and Ligler, F. S. (1991) Chemistry and technology of evanescent wave biosensors, in Biosensors with Fiberoptics (Wingard, L. B. and Wise, D. L.), Humana, Totowa, NJ, pp 111–138
Axelrod, D. (1989) Total internal reflection fluorescence microscopy. Meth. Cell Biol. 30, 245–270.
Kronick, M. N. and Little, W. A. (1975) A new immunoassay based on fluorescence excitation by internal reflection spectroscopy. J Immunol. Meth. 8, 235–240.
Hirshfield, T. E. and Block, M. J. (1984) Fluorescent immunoassay employing optical fiber in capillary tube. U.S. Patent No. 4,447,546
Ligler, F. S., Golden, J. P., Shriver-Lake, L. C, Ogert, R. A., Wijesuria, D., and Anderson, G. P. (1993) Fiber-optic biosensor for the detection of hazardous materials. Immunomethods 3, 122–127
Shriver-Lake, L. C., Ogert, R. A., and Ligler, F. S. (1993) A fiber-optic evanescent-wave immunosensor for large molecules. Sensors Actuators 11, 239–243.
Shriver-Lake, L. C., Breslin, K. A., Charles, P. T., Conrad, D. W., Golden, J. P., and Ligler, F. S. (1995) Detection of TNT in water using an evanescent wave fiber-optic biosensor. Anal Chem 34, 2431–2435.
Walczak, I. M., Love, W. F., Cook, T. A., and Slovacek, R. E. (1992) The application of evanescent wave sensing to a high-sensitivity fluoroimmunoassay. Biosensors BioeJectron. 7, 39–48
Oroszlan, P., Thommen, C., Wehrli, M, Duveneck, G., and Ehrat, M. (1993) Automated optical sensing system for biochemical assays: a challenge for ELISA? Anal. Meth. Instrument. 1, 43–51.
Christensen, D., Johannson, T., and Petelenz, D. (1994) Biosensor development at the University of Utah. IEEE Engineer Med Biol. 13, 388–395.
Reichert, W. M., Ives, J. T., Suci, P. A., and Hlady, V. (1987) Excitation of fluorescent emission from solutions at the surface of polymer thin-film waveguides, an integrated optics technique for the sensing of fluorescence at the polymer/solution interface. Appl. Spec 41, 636–640.
Anderson, G. P., Golden, J. P., and Ligler, F. S(1993) A fiber optic biosensorcombination tapered fibers designed for improved signal acquistion. Biosensors Bioelectron. 8, 249–256.
Golden, J. P., Anderson, G. P., Rabbany, S. Y., and Ligler, F. S. (1994) An evanescent wave biosensor—part II: fluorescent signal acquisition from tapered fiber optic probes. IEEE Trans. Biomed Engineer. 41, 585–591.
Bhatia, S. K., Shriver-Lake, L. C., Prior, K. J., Georger, J. H., Calvert, J. M., Bredehorst, R., and Ligler, F. S. (1989) Use of thiol-terminal silanes and heterobifunctional crosshnkers for immobilization of antibodies on silica surfaces Anal Biochem. 178, 408–413.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Wadkins, R.M., Ligler, F.S. (1998). Immunobiosensors Based on Evanescent Wave Excitation. In: Rogers, K.R., Mulchandani, A. (eds) Affinity Biosensors. Methods in Biotechnology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-539-5:77
Download citation
DOI: https://doi.org/10.1385/0-89603-539-5:77
Publisher Name: Humana Press
Print ISBN: 978-0-89603-539-3
Online ISBN: 978-1-59259-485-6
eBook Packages: Springer Protocols