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Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments

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Capillary Electrophoresis of Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 162))

Abstract

Field-inversion capillary electrophoresis (FICE) provides many of the same benefits as the more familiar, field-inversion gel electrophoresis (FIGE). In the capillary format, field inversion allows size separation of linear dsDNA into the megabase region. Whereas FIGE separation of megabase pair (Mb) DNA takes 24 h or more, the most rapid FICE separations are complete within 4 min (1,2). As with any capillary electroseparation, the technique is rapid and provides high resolution, but is analytical scale only. Preparative scale use of capillary electrophoresis (CE) is not very practical, although apparatus capable of the collection of CE fractions have been developed (3).

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Michigan, Ann Arbor, MI

    Michael D. Morris & Olivia de Carmejane

  2. Department of Biochemistry, University of Minnesota, St. Paul, MN

    Jeffrey J. Schwinefus

Authors
  1. Michael D. Morris
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  2. Jeffrey J. Schwinefus
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  3. Olivia de Carmejane
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Editor information

Editors and Affiliations

  1. Australian Genome Research Facility, University of Queensland, Brisbane

    Keith R. Mitchelson

  2. Walter & Eliza Hall Institute, Parkville, Australia

    Keith R. Mitchelson

  3. Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

    Jing Cheng

  4. Aviva Biosciences Corporation, San Diego, CA

    Jing Cheng

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© 2001 Humana Press Inc.

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Morris, M.D., Schwinefus, J.J., de Carmejane, O. (2001). Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:307

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  • DOI: https://doi.org/10.1385/1-59259-055-1:307

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-779-3

  • Online ISBN: 978-1-59259-055-1

  • eBook Packages: Springer Protocols

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