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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 40))

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Abstract

Flow cytometry, as the name suggests, is the analysis of cells (which carry one or more fluorescent labels) moving in a fluid flow (1,2). This technique has become widely used because of the enormous increase in the number and range of specificities of antibodies to cell determinants. Monoclonal and polyclonal antibodies to both murine and human antigens, indeed antibodies that have a fluorochrome covalently attached (and which have already been tested in various assay systems), are readily commercially available.

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References

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Further Reading

  • Shapiro, H. M. (1988) Practial Flow Cytometry, 3rd ed., Wiley-Liss Inc., New York. The Cytometry Journal.

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© 2000 Humana Press Inc.

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McKay, P.F. (2000). Flow Cytometric Analysis. In: George, A.J.T., Urch, C.E. (eds) Diagnostic and Therapeutic Antibodies. Methods in Molecular Medicine, vol 40. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-076-4:407

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  • DOI: https://doi.org/10.1385/1-59259-076-4:407

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-798-4

  • Online ISBN: 978-1-59259-076-6

  • eBook Packages: Springer Protocols

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