Abstract
Sendai virus (SeV) is an enveloped virus with a nonsegmented negativestrand RNA genome of 15,384 nucleotides; it is a member of the Paramyxoviridae family (1–3). The virus is pneumotropic in rodent species such as mice and rats, but no pathogenicity has been reported in humans. SeV has been utilized for generation of hybrid cells between mammalian cells of different species in vitro by use of its cell fusion activity, and it has contributed to somatic cell genetics (4). The inactivated SeV has been also used for generation of a fusogenic viral liposome (hemagglutinating virus of Japan, or Sendai virus [HVJ]-liposome), which aimed to improve the efficiency of liposome-mediated DNA transfer (5,6).
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References
Lamb R. A. and Kolakofsky D. (1996) Paramyxoviridae: the viruses and their replication,. In Virology, 3rd ed. (Fields B. N., Knipe D. M., and Howley P. M., eds.), Lippincott-Raven Philadelphia, pp. 1177–1204.
Conzelmann K.-K. (1998) Nonsegmented nagative-strand RNA viruses: Genetics and manipulation of viral genomes. Ann. Rev. Genet. 32, 123–162.
Nagai Y. and Kato A. (1999) Paramyxovirus reverse genetics is coming of age. Microbiol. Immunol. 43, 613–624.
Okada Y. and Tadokoro J. (1962) Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich’s ascites tumor cells. Exp. Cell. Res. 26, 98–128.
Kato K., Nakanishi M., Kaneda Y., Uchida T., and Okada Y. (1991) Expression of hepatitis B virus surface antigen in adult rat liver. J. Biol. Chem. 26, 3361–3364.
Dzau V., Mann M., Morishita R., and Kaneda Y. (1996) Fusigenic viral lipo-some for gene therapy in cardiovascular diseases. Proc. Natl.Acad. Sci. USA 93, 11421–11425.
Kato A., Sakai Y., Shioda T., et al. (1996) Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense. Genes Cells 1, 569–579.
Garcin D., Pelet T., Calain P., et al. (1995) A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA. EMBO J. 14, 5773–5784.
Hasan M. K., Kato A., Shioda T., et al. (1997) Creation of an infectious recombinant Sendai virus expressing the firefly luciferase from 3′ proximal first locus. J. Gen. Virol. 78, 2813–2820.
Moriya C., Shioda T., Tashiro K., et al. (1998) Large quantity production with extreme convenience of human SDF-1α and SDF-1β by a Sendai virus vector. FEBSLett. 425, 105–111.
Yu D., Shioda T., Kato A. et al. (1997) Sendai virus-based expression of HIV-1 gp120: reinforcement by the V(−) version. Genes Cells 2, 457–466.
Sakai Y., Kiyotani K., Fukumura M., et al. (1999) Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication. FEBS Lett. 456, 221–226.
Toriyoshi H., Shioda T., Sato H., et al. (1999) Sendai virus-based production of HIV type 1 subtype B and subtype E envelope glycoprotein 120 antigen and their use for highly sensitive detection of subtype-specific serum antibody. AIDS Res. and Hum. Retroviruses 12, 1109–1120.
Yonemitsu Y., Kitson C., Ferrari S., et al. (2000) Efficient gene transfer to airway epithelium using recombinant Sendai virus. Nat. Biotechnol. 18, 970–973.
Li H-O., Zhu Y-H., Asakawa M., et al. (2000) A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression. J. Virol. 74, 6564–6569.
Shioda T., Iwasaki K., and Shibuta H. (1986) Determination of the complete nucleotide sequence of Sendai virus genome RNA and the predicted amino acid sequence of the F, HN, and L proteins. Nucleic Acids Res. 14, 1545–1563.
Calain P. and Roux L. (1993) The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. J. Virol. 67, 4822–4830.
Korakofsky D., Pelet T., Garcin D., et al. (1998) Paramyovirus RNA synthesis and requirement for hexamer genome length: the rule of six revisited. J. Virol. 72, 891–899.
Arai T., Matsumoto K., Saitoh K., et al. (1998) A new system for stringent, high-titer vesicular stomatitis virus G protein-pseudotyped retrovirus vector induction by introduction of Cre recombinase into stable prepackaging cell lines. J. Virol. 72, 1115–1121.
Kanegae Y., Takamori K., Sato Y., et al. (1996) Efficient gene activation system on mammalian cell chromosome using recombinant adenovirus producing Cre recombinase. Gene 181, 207–212.
Fuerst T. R., Niles E. G., Studier F. W., and Moss B. (1986) Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83, 8122–8126.
Tsung K., Yim J. H., Marti W., Buller R. M. L., and Norton J. A. (1996) Gene expression and cytopathic effect of vaccinia virus inactived by psoralen and longwave UV light. J Virol. 70, 165–171.
Segawa H., Kato M., Yamashita T., and Taira H. (1998) The role of individual cysteine residues of Sendai virus fusion protein in intracellular transport. J. Biochem. 123, 1064–1072.
Curran J., Boeck R., and Kolakofsky D. (1991) The Sendai virus P gene expresses both an essential protein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO J. 10, 3079–3085.
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Iida, A., Hasegawa, M. (2002). Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:361
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DOI: https://doi.org/10.1385/1-59259-141-8:361
Publisher Name: Springer, Totowa, NJ
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