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Selection of Glycosaminoglycan-Deficient Mutants

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Proteoglycan Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 171))

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Abstract

Mutant cell lines provide an excellent model for studying the structure, assembly and function of proteoglycans under the controlled conditions of tissue culture. Numerous proteoglycan-deficient strains have been isolated, mostly in Chinese hamster ovary cells, and in many cases the defects have been characterized both genetically and biochemically (see Table 1). Biochemical analysis of the mutants has confirmed that various enzyme activities detected in cell-free extracts using synthetic substrates actually play a role in proteoglycan assembly in vivo. The cell lines have allowed investigators to study how altering the composition of proteoglycans affects fundamental properties of cells, such as adhesion and signaling. Moreover, animal cell mutants provide the background for predicting the phenotype of organismal mutants defective in proteoglycan assembly.

Table 1 Cell Mutants with Defined Defects in Glycosaminoglycan Biosynthesis
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Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, CA

    Xiaomei Bai, Brett Crawford & Jeffrey D. Esko

Authors
  1. Xiaomei Bai
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  2. Brett Crawford
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  3. Jeffrey D. Esko
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Editor information

Editors and Affiliations

  1. Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA

    Renato V. Iozzo MD

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© 2001 Humana Press Inc., Totowa, NJ

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Bai, X., Crawford, B., Esko, J.D. (2001). Selection of Glycosaminoglycan-Deficient Mutants. In: Iozzo, R.V. (eds) Proteoglycan Protocols. Methods in Molecular Biology™, vol 171. Humana Press. https://doi.org/10.1385/1-59259-209-0:309

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  • DOI: https://doi.org/10.1385/1-59259-209-0:309

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-759-5

  • Online ISBN: 978-1-59259-209-8

  • eBook Packages: Springer Protocols

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