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In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes

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Gene Knockout Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 158))

Abstract

Embryonic stem (ES) cells, the totipotent cells of early embryos established as permanent lines (1,2) retain their developmental capacity in vivo (3) as well as in vitro (see 4–6). The totipotent properties of ES cells are the basis of the gene-targeting technology to create mutant mice strains with inactivated genes by homologous recombination (7).

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Author information

Authors and Affiliations

  1. In Vitro Differentiation Group, IPK Gatersleben, Gatersleben, Germany

    Anna M. Wobus, Kaomei Guan & Uta Pich

Authors
  1. Anna M. Wobus
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  2. Kaomei Guan
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  3. Uta Pich
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Editor information

Editors and Affiliations

  1. National Vision Research Institute of Australia, Carlton, Victoria, Australia

    Martin J. Tymms

  2. Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia

    Ismail Kola

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© 2001 Humana Press Inc.

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Wobus, A.M., Guan, K., Pich, U. (2001). In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:263

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  • DOI: https://doi.org/10.1385/1-59259-220-1:263

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-572-0

  • Online ISBN: 978-1-59259-220-3

  • eBook Packages: Springer Protocols

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