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Extraction of Ancient DNA

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PCR Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 226))

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Abstract

The DNA extraction process represents one of the critical stages in the analysis of degraded or ancient DNA. If polymerase chain reaction (PCR) amplification starts from a poor extract containing low template quantities, stochastic variation in the amplification of individual alleles may lead to allelic dropout (1), resulting in a high risk of false-homozygous typing of a heterozygous sample (2).

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References

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Author information

Authors and Affiliations

  1. Paleo DNA Laboratory, Department of Anthropology, Lakehead University, Thunder Bay, Ontario, Canada

    Wera M. Schmerer

Authors
  1. Wera M. Schmerer
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Editor information

Editors and Affiliations

  1. Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK

    John M. S. Bartlett

  2. Department of Hematology, Royal Infirmary of Edinburgh, Edinburgh, UK

    David Stirling

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© 2003 Humana Press Inc.

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Schmerer, W.M. (2003). Extraction of Ancient DNA. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:57

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  • DOI: https://doi.org/10.1385/1-59259-384-4:57

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-642-0

  • Online ISBN: 978-1-59259-384-2

  • eBook Packages: Springer Protocols

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