Abstract
Compared to classical two-dimensional electrophoresis (2-DE) with carrier ampholytes (1,2), the advent of 2-DE with immobilized pH gradients (IPG-Dalt) (3) has produced significant improvements in 2-D electrophoretic separation with respect to:
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1.
Higher resolution by using well-defined narrow-pH gradients (3).
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2.
Improved reproducibility, as was demonstrated by interlaboratory comparisons (4,5).
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3.
Higher loading capacity for micropreparative runs, which has accelerated spot identification by microsequencing and mass spectrometry (6–9).
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4.
The possibility to resolve—under steady-state conditions—alkaline proteins, which are normally lost by the cathodic drift of carrier ampholyte focusing or separated by nonequilibrium pH gradient electrophoresis (NEPHGE) with limited reproducibility (10).
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© 1999 Humana Press Inc., Totowa, NJ
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Görg, A. (1999). IPG-Dalt of Very Alkaline Proteins. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:197
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DOI: https://doi.org/10.1385/1-59259-584-7:197
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