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Preparing 2-D Protein Extracts from Caenorhabditis elegans

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2-D Proteome Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 112))

Abstract

The small nematode Caenorhabditis elegans (C. elegans, 1 mm in length) originally was introduced into the laboratory in 1965 by Brenner (1) and has since become an important model organism. Caenorhabditis elegans for a variety of reasons offers excellent conditions for the study of basic features of life (2). C. elegans is surrounded by a rigid cuticula, which is, however, completely transparent. The adult organism is composed of a constant number of somatic cells (959 in the adult hermaphrodite, 1031 in the adult male), of which the complete cell lineage during development is known in all details (3). Altogether, this makes C. elegans a very suitable model organism to approach fundamental problems in development and aging.

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References

  1. Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71–94.

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  2. Wood, W. B. (ed.) (1988) The nematode Caenorhabditis elegans. Cold Spring Harbor Monograph Ser. No. 17, pp. 1–16.

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  3. Sulston, J. and Hodgkin, J. (1988) The nematode Caenorhabditis elegans. Cold Spring Harbor Monographs Ser. No. 17, 587–590.

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  4. Bini, L., Heid, H., Liberatori, S., Geier, G., Pallini, V., and Zwilling, R. (1997) Two-dimensional gelelectrohphoresis of Caenorhabditis elegans homogenates and identification of protein spots by micro-sequencing. Electrophoresis 18, 557–562.

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  5. Epstein, H. F. and Shakes, D. G. (eds.) (1995) Caenorhabditis elegans: Modern biological analysis of an organism. Methods in Cell Biology, vol. 48, Academic, San Diego, pp. 4–27.

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  6. Kruger, N. J. (1994) The Bradford Method for Protein Quantitation. Methods in Molecular Biology, vol. 32, Basic Protein and Peptide Protocols. Humana Press Inc., Totowa, NJ, pp. 9–15.

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© 1999 Humana Press Inc., Totowa, NJ

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Zwilling, R. (1999). Preparing 2-D Protein Extracts from Caenorhabditis elegans . In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:43

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  • DOI: https://doi.org/10.1385/1-59259-584-7:43

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-524-9

  • Online ISBN: 978-1-59259-584-6

  • eBook Packages: Springer Protocols

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