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In Vitro Replication of Chromatin Templates

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Chromatin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 119))

Abstract

Eukaryotic genome replication implies that both the DNA and the associated proteins must be duplicated during each S phase. Model systems such as the simian virus 40 (SV40) in vitro replication system have provided important information on the mechanism of DNA replication in mammalian cells (reviewed in ref. 1). The SV40 genome is replicated by the host replication machinery in conjunction with a single viral protein, T antigen (T-Ag). Replication of the viral genome begins at the well-defined origin and proceeds in a bidirectional and semidiscontinuous manner. A valuable feature is that SV40 DNA exists in infected cells as minichromosomes which resemble the host cell chromatin. Therefore studies on SV40 DNA replication can address issues such as chromatin replication.

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Author information

Authors and Affiliations

  1. Division of Biology, University of Konstanz, Konstanz, Germany

    Claudia Gruss

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  1. Claudia Gruss
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Editor information

Editors and Affiliations

  1. Adolf-Butenandt-Institut-Molekularbiologie, Ludwig-Maximilians-Universität, München, Germany

    Peter B. Becker

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© 1999 Humana Press Inc.

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Gruss, C. (1999). In Vitro Replication of Chromatin Templates. In: Becker, P.B. (eds) Chromatin Protocols. Methods in Molecular Biology™, vol 119. Humana Press. https://doi.org/10.1385/1-59259-681-9:291

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  • DOI: https://doi.org/10.1385/1-59259-681-9:291

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-665-9

  • Online ISBN: 978-1-59259-681-2

  • eBook Packages: Springer Protocols

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