Abstract
Post-transcriptional gene silencing is a powerful tool to reveal gene function. In this chapter, we have used gene-array technology to analyze changes in gene expression after specific targeting of the protein kinase Cα isoform with small interfering RNAs (siRNAs). Expression profiles were investigated on a human gene array of 588 key genes involved in DNA synthesis, apoptosis, cell-cell communication, intracellular signal-transduction pathways, cell-surface molecules and transcription factors. The data indicate that siRNAs are an ideal tool for target validation, and show that RNA-dependent RNA polymerase is not required for RNAi in mammalian cells.
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© 2004 Humana Press Inc., Totowa, NJ
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Leirdal, M., Sioud, M. (2004). Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKCα siRNA. In: Sioud, M. (eds) Ribozymes and siRNA Protocols. Methods in Molecular Biology™, vol 252. Humana Press. https://doi.org/10.1385/1-59259-746-7:493
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DOI: https://doi.org/10.1385/1-59259-746-7:493
Publisher Name: Humana Press
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