Abstract
Basic techniques for fluorescence in situ hybridization (FISH) mapping that have been used in genome projects on schistosomes and filariae are introduced. The chapter shows techniques specific for bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones and includes experiences of chromosome preparation, DNA labeling, hybridization, microscopy, and localization of BAC clones.
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© 2004 Humana Press Inc., Totowa,NJ
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Hirai, H., Hirai, Y. (2004). FISH Mapping for Helminth Genome. In: Melville, S.E. (eds) Parasite Genomics Protocols. Methods in Molecular Biology™, vol 270. Humana Press. https://doi.org/10.1385/1-59259-793-9:379
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DOI: https://doi.org/10.1385/1-59259-793-9:379
Publisher Name: Humana Press
Print ISBN: 978-1-58829-062-5
Online ISBN: 978-1-59259-793-2
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