Abstract
Tissue culture refers to the in vitro growth or maintenance of organs, tissues, or cells. Methods of tissue culture in most cases have the dual goals of preservation of physical integrity and viability (1,1). As a nonreplicating tissue responsible for significant endocrine hormone production, islets of Langerhans represent a unique challenge for the tissue-culture laboratory. Physical integrity must be maintained not only on a cellular level, but also in terms of the three-dimensional (3D) matrix and multicellular composition of the intact islets. The maintenance of viability with respect to islet culture requires not only the preservation of cellular integrity, but also the retention of an ability to respond to external stimuli in a physiologically appropriate manner. Specifically, with respect to the suitability of tissue for islet transplantation, this refers to preserving the ability to produce and release insulin in response to secretagogues.
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References
Barnes, D. (1987) Serum-free animal cell culture. BioTechniques 5, 534–541.
Unchern, S. (1999) Basic techniques in animal cell culture. Drug Deliv. Syst. Workshop., August 19-20, 1999, Bangkok, Thailand.
Fraga, D. W., Sabek, O., Hathaway, D. K., and Gaber, A. O. (1998) A comparison of media supplement methods for the extended culture of human islet tissue. Transplantation 65(8), 1060–1066.
Gaber, A. O., Fraga, D. W., Callicut, C. S., Gerling, I. C., Sabek, O. M., and Kotb, M. Y. Improved in vivo pancreatic islet function after prolonged in vitro islet culture. Transplantation 72(11), 1730–1736.
Ricordi, C., Lacy, P. E., Finke, E. H., Olack, B. J., and Scharp, D. W. (1988) Automated method for isolation of human pancreatic islets. Diabetes 37, 413–420.
Lake, S. P., Bassett, P. D., Larkins, A., et al. (1989) Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator. Diabetes 38(Suppl. 1), 143.
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© 2005 Humana Press Inc.
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Fraga, D.W., Gaber, A.O., Kotb, M. (2005). Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media. In: Picot, J. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine™, vol 107. Humana Press. https://doi.org/10.1385/1-59259-861-7:303
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DOI: https://doi.org/10.1385/1-59259-861-7:303
Publisher Name: Humana Press
Print ISBN: 978-1-58829-222-3
Online ISBN: 978-1-59259-861-8
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