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In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion

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Placenta and Trophoblast

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 121))

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Abstract

Despite the importance of the definitive chorio-allantoic placenta in fetal survival, fetal development, and long-term health of the adult, little is known about how the placenta’s individual components, the allantois and the chorion, proliferate and develop. In this chapter, two techniques will be described: (1) explanting murine allantoises for culture in isolation, and (2) grafting murine allantoises into living whole mouse embryos. Together, these will enable study of differentiation of allantoic mesoderm into the umbilical vasculature, and the mechanism(s) by which the allantois unites with the chorion to form the chorio-allantoic placenta.

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References

  1. Georgiades, P., Ferguson-Smith, A. C., and Burton, G. J. (2002) Comparative developmental anatomy of the murine human definitive placentae. Placenta 23, 3–19.

    Article  PubMed  CAS  Google Scholar 

  2. Downs, K. M. (1998) The murine allantois. Curr. Top. Dev. Biol. 39, 1–33.

    Article  PubMed  CAS  Google Scholar 

  3. Gardner, R. L., Lyon, M. F., Evans, E. P., and Burtenshaw, M. D. (1985) Clonal analysis of X-chromosome inactivation and the origin of the germ line in the mouse embryo. J. Embryol. Exp. Morph. 52, 141–152.

    Google Scholar 

  4. Lawson, K. A., Meneses, J., and Pedersen, R. A. (1991) Clonal analysis of epiblast fate during germ layer formation in the mouse embryo. Development 113, 891–911.

    PubMed  CAS  Google Scholar 

  5. Downs, K. M., Hellman, E. R., McHugh, J., Barrickman, K., and Inman, K. (2004) Investigation into a role for the primitive streak in development of the murine allantois. Development 131, 37–55.

    Article  PubMed  CAS  Google Scholar 

  6. Sabin, F. R. (1920) Studies on the origin of blood-vessels and of red blood-corpuscles as seen in the living blastoderm of chicks during the second day of incubation. Contr. Embryol. 9, 215–262.

    Google Scholar 

  7. Downs, K. M., Gifford, S., Blahnik, M., and Gardner, R. L. (1998) The murine allantois undergoes vasculogenesis that is not accompanied by erythropoiesis. Development 125, 4507–4521.

    PubMed  CAS  Google Scholar 

  8. Downs, K.M., Temkin, R., Gifford, S., and McHugh, J. (2001) Study of the murine allantois by allantoic explants. Dev. Biol. 233, 347–364.

    Article  PubMed  CAS  Google Scholar 

  9. Gurtner, G. C., Davis, V., Li, H., McCoy, M. J., Sharpe, A., and Cybulsky, M. I. (1995) Targeted disruption of the murine VCAM1 gene: essential role of VCAM-1 in chorioallantoic fusion and placentation. Genes Dev. 9, 1–14.

    Article  PubMed  CAS  Google Scholar 

  10. Kwee, L., Baldwin, H. S., Shen, H. M., et al. (1995) Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development 121, 489–503.

    PubMed  CAS  Google Scholar 

  11. Carmeliet, P., Ferreira, V., Breier, G., et al. (1996) Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature 380, 435–439.

    Article  PubMed  CAS  Google Scholar 

  12. Downs, K. M. and Gardner, R. L. (1995) An investigation into early placental ontogeny: allantoic attachment to the chorion is selective and developmentally regulated. Development 121, 407–416.

    PubMed  CAS  Google Scholar 

  13. Yang, J. T., Rayburn, H., and Hynes, R. O. (1995) Cell adhesion events mediated by α4 integrins are essential in placental and cardiac development. Development 121, 549–560.

    PubMed  CAS  Google Scholar 

  14. Downs, K. M. (2002) Early placentation in the mouse. Placenta 23, 116–131.

    Article  PubMed  CAS  Google Scholar 

  15. Belaoussoff, M., Farrington, S. M., and Baron, M.H. (1998) Hematopoietic induction and respecification of A-P identity by visceral endoderm signaling in the mouse embryo. Development 125, 5009–5018.

    PubMed  CAS  Google Scholar 

  16. Wilt, F. H. (1965) Erythropoiesis in the chick embryo: the role of endoderm. Science 147, 1588–1590.

    Article  PubMed  CAS  Google Scholar 

  17. Friedrich, G. and Soriano, P. (1991) Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513–1523.

    Article  PubMed  CAS  Google Scholar 

  18. Downs, K. M. and Harmann, C. (1997) Developmental potency of the murine allantois. Development 124, 2769–2780.

    PubMed  CAS  Google Scholar 

  19. Champlin, A. K., Dorr, D. L., and Gates, A. H. (1973) Determining the stage of the estrous cycle in the mouse by the appearance of the vagina. Biol. Reprod. 8, 491–494.

    PubMed  CAS  Google Scholar 

  20. Downs, K. M. and Davies, T. (1993) Staging of gastrulation in mouse embryos by morphological landmarks in the dissection microscope. Development 118, 1255–1266.

    PubMed  CAS  Google Scholar 

  21. Cockroft, D. L. (1990) Dissection and culture of post-implantation mouse embryos, in Postimplantation Mammalian Embryos: A Practical Approach (Copp, A. J. and Cockroft, D. L., eds.). IRL, Oxford, UK: pp. 15–40.

    Google Scholar 

  22. Lawson, K. A., Meneses, J. J., and Pedersen, R. A. (1986) Cell fate and cell lineage in the endoderm of the presomite mouse embryo, studied with an intracellular tracer. Dev. Biol. 115, 325–339.

    Article  PubMed  CAS  Google Scholar 

  23. Lawson, K. A. and Pedersen, R. A. (1987) Cell fate, morphogenetic movement and population kinetics of embryonic endoderm at the time of germ layer formation in the mouse. Development 101, 627–652.

    PubMed  CAS  Google Scholar 

  24. Gardner, D. K. and Lane, M. (2000) Embryo culture systems, in Handbook of In Vitro Fertilization (Trounson, A. O. and Gardner, D. K., eds.). CRC, Boca Raton, FL: pp. 205–263.

    Google Scholar 

  25. Beddington, R. S. P. and Lawson, K. A. (1990) Clonal analysis of cell lineages, in Postimplantation Mouse Embryos: A Practical Approach (Copp, A. J. and Cockroft, D. L., eds.). IRL, Oxford, UK: pp. 267–292.

    Google Scholar 

  26. Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. R. (2003) Manipulating the Mouse Embryo: A Laboratory Manual, Third Edition Edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  27. Brown, N. A. (1990) Routine assessment of morphology and growth: scoring systems and measurements of size, in Postimplantation Mammalian Embryos: A Practical Approach (Copp, A. J. and Cockroft, D. L., eds.). IRL Press, Oxford, UK: pp. 93–108.

    Google Scholar 

  28. Brown, N. A. and Fabro, S. (1981) Quantitation of rat embryonic development in vitro: a morphological scoring system. Teratology 24, 65–78.

    Article  PubMed  CAS  Google Scholar 

  29. Beddington, R. S. P. (1987) Isolation, culture and manipulation of post-implantation mouse embryos, in Mammalian Development: A Practical Approach (Monk, M., ed.). IRL, Oxford, UK: pp. 43-70.

    Google Scholar 

  30. Kaufman, M. H. (1992) The Atlas of Mouse Development. Academic, London, UK.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Anatomy, University of Wisconsin-Madison Medical School, Madison, WI

    Karen M. Downs

Authors
  1. Karen M. Downs
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Editor information

Editors and Affiliations

  1. Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS

    Michael J. Soares

  2. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS

    Joan S. Hunt (University Distinguished Professor, Vice Chancellor for Research) (University Distinguished Professor, Vice Chancellor for Research)

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© 2006 Humana Press Inc.

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Downs, K.M. (2006). In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicine™, vol 121. Humana Press. https://doi.org/10.1385/1-59259-983-4:239

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  • DOI: https://doi.org/10.1385/1-59259-983-4:239

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-404-3

  • Online ISBN: 978-1-59259-983-7

  • eBook Packages: Springer Protocols

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