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Discovery of Differentially Expressed Genes

Technical Considerations

  • Protocol
Target Discovery and Validation Reviews and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 360))

Summary

Identification and characterization of differentially expressed genes may be an important first step toward the understanding of both normal physiology and disease. A multitude of techniques belonging to two main categories have been developed to identify the differences in gene expression between samples from different biological origin: selection techniques and global techniques. Whereas the selection techniques strive to identify specific differentially expressed genes, the global techniques analyze the total transcriptome or a major part of the RNA population in a defined biological material. By exploiting the known sequences of the adaptors used in suppressive subtraction hybridization technique, a strategy named novel rescue—suppression-subtractive hybridization was developed. It should facilitate the discovery of differentially expressed genes.

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Authors and Affiliations

  1. Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    Øystein Røsok & Mouldy Sioud

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  1. Øystein Røsok
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  2. Mouldy Sioud
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Editors and Affiliations

  1. Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    Mouldy Sioud

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© 2007 Humana Press Inc.

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Røsok, Ø., Sioud, M. (2007). Discovery of Differentially Expressed Genes. In: Sioud, M. (eds) Target Discovery and Validation Reviews and Protocols. Methods in Molecular Biology™, vol 360. Humana Press. https://doi.org/10.1385/1-59745-165-7:115

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  • DOI: https://doi.org/10.1385/1-59745-165-7:115

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-656-6

  • Online ISBN: 978-1-59745-165-9

  • eBook Packages: Springer Protocols

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