Abstract
The unique fluorescence properties of quantum dots (QDs), particulary their large extinction coefficients and photostability, make them ideal probes for tracking proteins in live cells using real-time visualization. We have shown that QDs conjugated to epidermal growth factor act as functional ligands for their receptor, erbB1. Here, we describe protocols for (1) conjugation of streptavidin-QDs to biotinylated ligand, (2) formation of ligand-QD-receptor complexes, and (3) quantification of binding and internalization of receptor complex using both high-resolution fluorescence microscopy and flow cytometry.
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© 2007 Humana Press Inc., Totowa, NJ
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Lidke, D.S., Nagy, P., Jovin, T.M., Arndt-Jovin, D.J. (2007). Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors. In: Bruchez, M.P., Hotz, C.Z. (eds) Quantum Dots. Methods in Molecular Biology, vol 374. Humana Press. https://doi.org/10.1385/1-59745-369-2:69
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DOI: https://doi.org/10.1385/1-59745-369-2:69
Publisher Name: Humana Press
Print ISBN: 978-1-58829-562-0
Online ISBN: 978-1-59745-369-1
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