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In Situ Hybridization

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Handbook of Neurochemistry and Molecular Neurobiology

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Abstract:

In situ hybridisation is a powerful technique for determining the distribution of specific mRNA species in tissues such as brain that comprise different cell types. Its utility in molecular neurobiology has driven the development of a variety of variations of this technique will be reviewed.

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Abbreviations

BCIP:

5-bromo-4-chloro-3-indolyl phosphate, toluidine salt

CCD:

charged-coupled device

CNS:

central nervous system

cDNA:

copy or complimentary RNA

cRNA:

copy or complimentary RNA

DNase:

deoxyribonuclease

FITC:

fluorescein isothiocyanate

mRNA:

messenger RNA

NBT:

nitro blue tetrazolium choride

PCR:

polymerase chain reaction

RNase:

ribonclease

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Authors
  1. N. Bateson Alan
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Editors and Affiliations

  1. Center for Neurochemistry, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, New York, 10962, USA

    Abel Lajtha (Director) (Director)

  2. Department of Pharmacology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada

    Glenn Baker , Susan Dunn  & Andrew Holt ,  & 

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© 2007 Springer Science+Business Media, LLC

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Alan, N.B. (2007). In Situ Hybridization. In: Lajtha, A., Baker, G., Dunn, S., Holt, A. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-30401-4_16

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