Introduction
A major goal in studying microbial ecology has been to understand the interaction between species in a community and their environment. Different bottom-up and top-down approaches have been applied to tackle this challenge. Top-down approaches, most notably metagenomics, target the community as a whole but have limited resolution at the species or strain level in spite of its high-throughput and comprehensive coverage. In addition, the metagenomics approach is often unable to provide a comprehensive assessment of individual members within the community. Complementary to the top-down approaches, bottom-up approaches, such as isolation and culture methods targeting individual cells, can decipher genomic information from individual bacteria cells, thus offering insight into cellular mechanisms from highly specific, rare bacteria subspecies. One major technical challenge of bottom-up approaches is the difficulty in obtaining pure laboratory cultures for genomic, proteomic, and...
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Chiang, HI., Chen, C.H., Lo, YH. (2013). Integrated Lab-on-Chip System, Genome Sequencing of Single Microbial Cells, Project. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_85-4
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DOI: https://doi.org/10.1007/978-1-4614-6418-1_85-4
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