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Structural Elucidation of Glycerolipids (TGs, GPLs) by Vacuum MALDI HE-CID TOF/RTOF Mass Spectrometry

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Encyclopedia of Lipidomics

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In terms of mass spectrometric analysis, glycerolipids (GLs) can be divided into two major compound classes, triacylglycerols (TGs) and glycerophospholipids (GPLs). The latter group can be subdivided into glycerophosphatidic acids (GPA), glycerophosphatidylserine (GPS), glycerophosphatidylethanolamine (GPE), glycerophosphatidylcholine (GPC), glycerophosphatidylglycerol (GPG), glycerophosphatidylinositol (GPI), and the dimeric GPL cardiolipin. Under vacuum MALDI conditions, glycerolipids form either [M-H]−, [M + H]+, or various types of (multiply) sodiated species (even without the addition of sodium salts) depending on their particular structure. For structural elucidation high-energy collision-induced dissociation in combination with tandem time-of-flight mass spectrometry (HE-CID TOF/RTOF-MS) at high collision energies (ELAB = 20 keV) offers the most complete fragmentation across the recorded m/z range in one step of all tandem mass spectrometric techniques currently available.

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References

  • Cheng C, Gross ML, Pittenauer E. Complete structural elucidation of triacylglycerols by tandem sector mass spectrometry. Anal Chem. 1998;70:4417–26. doi:10.1021/ac9805192.

    Article  CAS  PubMed  Google Scholar 

  • Kubo A, Satoh T, Itoh Y, Hashimoto M, Tamura J, Cody RB. Structural analysis of triacylglycerols by using a MALDI-TOF/TOF system with monoisotopic precursor selection. J Am Soc Mass Spectrom. 2013;24:684–9. doi:10.1007/s13361-012-0513-9.

    Article  CAS  PubMed  Google Scholar 

  • Pittenauer E, Allmaier G. The renaissance of high-energy CID for structural elucidation of complex lipids: MALDI-TOF/RTOF-MS of alkali cationized triacylglycerols. J Am Soc Mass Spectrom. 2009;90:1037–47. doi:10.1016/j.jasms.2009.01.009.

    Article  Google Scholar 

  • Pittenauer E, Allmaier G. A universal product ion nomenclature for [M-H](−), [M + H](+) and [M + nNa-(n-1)H](+) (n = 1-3) glycerophospholipid precursor ions based on high-energy CID by MALDI-TOF/RTOF mass spectrometry. Int J Mass Spectrom. 2011;301:90–101. doi:10.1016/j.ijms.2010.07.012.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria

    Ernst Pittenauer & Günter Allmaier

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  1. Ernst Pittenauer
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  2. Günter Allmaier
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Correspondence to Ernst Pittenauer .

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Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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Pittenauer, E., Allmaier, G. (2017). Structural Elucidation of Glycerolipids (TGs, GPLs) by Vacuum MALDI HE-CID TOF/RTOF Mass Spectrometry. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_32-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_32-1

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  • Print ISBN: 978-94-007-7864-1

  • Online ISBN: 978-94-007-7864-1

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