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Single Cell Genomics

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Handbook of Single Cell Technologies

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Abstract

Single cells are the minimum physiological and biological units of tissues and organs. It is critical to isolate individual cells from tissues and characterize them at distinct molecular levels in order to understand the status and fate of each individual cell, their role within the organism, and their cell-to-cell interactions. During the past decade, technical advances in whole genome amplification (WGA) have enabled massive DNA sequencing at the single cell level, and this approach has been extensively applied to study physiological conditions in normal tissues, disease, and development. Single cell genomics was intensively developed to dissect intra-tissue heterogeneity, which is a very powerful tool for uncovering genomic diversity, particularly in cancer. Likewise, epigenetic analysis has been carried out at a single cell level and has provided new insights into cellular variation and refined cell-cell interactions and provided a comprehensive epigenetic atlas of cellular states and lineages. In this chapter, technical improvements in DNA amplification methods and emerging high-dimensional bioinformatics tools will be broadly described with an eventual discussion of perspectives in single cell genomics.

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Acknowledgments

This work was supported by MEXT KAKENHI (Grant-in-Aid for Young Scientists (A); grant number: 17H04991) and Research grant from The Naito Foundation.

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Authors and Affiliations

  1. Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan

    Yusuke Yamamoto, Anna Sanchez Calle & Takahiro Ochiya

Authors
  1. Yusuke Yamamoto
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  2. Anna Sanchez Calle
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  3. Takahiro Ochiya
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Correspondence to Yusuke Yamamoto .

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Editors and Affiliations

  1. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Tuhin Subhra Santra

  2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan

    Fan-Gang Tseng

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Yamamoto, Y., Calle, A.S., Ochiya, T. (2018). Single Cell Genomics. In: Santra, T., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_11-1

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  • DOI: https://doi.org/10.1007/978-981-10-4857-9_11-1

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  • Print ISBN: 978-981-10-4857-9

  • Online ISBN: 978-981-10-4857-9

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