Abstract
A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture of Iscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSA/oleic acid complex. Several myelomas, hybridomas derived from different myelomas and spleen cells, and other lymphoid and non-lymphoid cell lines were cultivated at growth rates comparable to those observed using serum-supplemented media. There was furthermore no reduction in the formation of products such as monoclonal antibodies or recombinant human interleukin-2.
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Abbreviations
- Ag8:
-
Mouse myeloma cell line P3-X63-Ag8.653
- BME:
-
Basal Medium Eagle
- BSA:
-
Bovine Serum Albumin
- DMEM:
-
Dulbecco's Modified Eagle's Medium
- EDTA:
-
Ethylenediaminete-traacetic Acid
- e-PC:
-
Phosphatidyl choline from egg yolk
- FCS:
-
Fetal Calf Serum
- FGF:
-
Fibroblast Growth Factor
- GHL:
-
Glycyl-histidyl-lysine
- HDL:
-
High Density Lipoprotein
- HPL:
-
Human Plasma Lipid
- IF:
-
1:1 mixture of IMDM and Ham's F12
- IMDM:
-
Iscove's Modified Dulbecco's medium
- LDL:
-
Low Density Lipoprotein
- NS1:
-
Mouse myeloma cell line NSI-1-Ag4-1
- PBS:
-
Phosphate Buffered Saline
- s-PC:
-
Phosphatidylcholine from soy beans
- s-PE:
-
Phosphatidylethanolamine from soy beans
- s-lecithin:
-
lecithin from soy beans
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Jäger, V., Lehmann, J. & Friedl, P. Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors. Cytotechnology 1, 319–329 (1988). https://doi.org/10.1007/BF00365077
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DOI: https://doi.org/10.1007/BF00365077