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Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum

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Abstract

Mechanisms of photoadaptation of photosynthesis have been studied in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum. Algal strains isolated from the clam Tridacna maxima, the sea anemone Aiptasia pulchella, and the scleractinian coral Montipora verrucosa were maintained in the defined medium ASP-8A, and were grown at irradiances ranging from 22 to 248 μE m-2 s-1 on a 14 h:10 h (light:dark) photoperiod at 26°C. All algal cultures were analysed during log-phase of growth. At all light levels, rates of cell division and photosynthesis were determined, as were cell volumes, pigmentation (including chlorophyll a, chlorophyll c 2, peridinin, β-carotene and xanthophylls), and carbon and nitrogen content. Low light-induced changes in pigmentation were evident to varying degrees in all three algal strains, although alterations in the photosynthesis-irradiance relations were distinctly different in each strain. The algae from T. maxima show the least photoadaptive capability, and seem to photoadapt by changing photosynthetic unit (PSU) size. Algae from A. pulchella appear to adapt by changing PSU number, while algae from M. verrucosa appear to photoadapt by changes in the activities of CO2-fixing enzymes or electron transport systems. These are the first observations that demonstrate functional differences in different strains of S. microadriaticum. The adaptive capabilities of the algae appear to correlate well with the ecological distribution of their respective hosts. The study was made from July 1981 through December 1982.

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Literature cited

  • Ahles, M. D.: Some aspects of the morphology and physiology of Symbiodinium microadriaticum, 158 pp. Ph.D. thesis, Fordham University, New York 1967

    Google Scholar 

  • Berseneva, G. P., L. M. Sergeeva and Z. Z. Fineko: Marine plankton algae adaptation to light. Okeanologija, Mosk. 18, 298–306 (1978)

    Google Scholar 

  • Brand, L. E. and R. R. L. Guillard: The effects of continuous light and light intensity on the reproduction rates of twenty-two species of marine phytoplankton. J. exp. mar. Biol. Ecol. 50, 119–132 (1981)

    Article  Google Scholar 

  • Brown, T. E. and F. L. Richardson: The effects of growth environment on the physiology of algae: light intensity. J. Phycol. 4, 38–54 (1968)

    Google Scholar 

  • Chan, A. T.: Comparative physiological study of marine diatoms and dinoflagellates in relation to irradiance and cell size. I. Growth under continuous light. J. Phycol. 14, 396–402 (1978)

    Google Scholar 

  • Coles, S. L. and P. L. Jokiel: Synergistic effects of temperature, salinity and light on the hermatypic coral Montipora verrucosa. Mar. Biol. 49, 187–195 (1978)

    Google Scholar 

  • Culberson, C., R. M. Pitkowicz and J. E. Hawley: Seawater alkalinity determination by the pH method. J. mar. Res. 28, 15–21 (1970)

    Google Scholar 

  • Drew, E. A.: The biology and physiology of alga-invertebrate symbioses. I. The density of symbiotic algal cells in a number of hermatypic hard corals and alcyonarians from various depths. J. exp. mar. Biol. Ecol. 9, 71–75 (1972)

    Article  Google Scholar 

  • Dustan, P.: Distribution of zooxanthellae and photosynthetic chloroplast pigments of the reef-building coral Montastrea annularis Ellis and Solander in relation to depth of a West Indian coral reef. Bull. mar. Sci. 29, 79–95 (1979)

    Google Scholar 

  • Dustan, P.: Depth-dependent photoadaptation by zooxanthellae of the reef coral Montastrea annularis. Mar. Biol. 68, 253–264 (1982)

    Google Scholar 

  • Falkowski, P. G. and Z. Dubinsky: Light-shade adaptation of Stylophora pistillata, a hermatypic coral from the Gulf of Eilat. Nature, Lond. 289, 172–175 (1981)

    Google Scholar 

  • Fitt, W. K. and R. K. Trench: The relation of diel patterns of cell division to diel patterns of motility in the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal). New Phytol. 94, 421–432 (1983)

    Google Scholar 

  • Glider, W. C., D. W. Phipps and R. L. Pardy: Localization of symbiotic dinoflagellate cells within tentacle tissue of Aiptasia pallida (Coelenterata, Anthozoa). Trans. Am. microsc. Soc. 99, 426–438 (1980)

    Google Scholar 

  • Goreau, T. F.: Calcium carbonate deposition by coralline algae and corals in relation to their roles as reef-builders. Ann. N.Y. Acad. Sci. 109, 127–167 (1963)

    PubMed  Google Scholar 

  • Houck, J.: The potential utilization of scleractinian coral in the study of marine environments, 199 pp. Ph.D. thesis, University of Hawaii, Honolulu 1978

    Google Scholar 

  • Jeffrey, S. W.: New spectrophotometric equations for determining chlorophylls a, b, c and c 2 in phytoplankton and higher plants. Rep. mar. Biochem. Unit C.S.I.R.O. 1974, 6–8 (1974)

    Google Scholar 

  • Jeffrey, S. W.: An improved thin-layer chromatographic technique for marine phytoplankton pigments. Limnol. Oceanogr. 26, 191–197 (1981)

    Google Scholar 

  • Jokiel, P. L. and R. H. York: Solar ultraviolet photobiology of the reef coral Pocillopora damicornis and symbiotic zooxanthellae. Bull. mar. Sci. 32, 301–315 (1982)

    Google Scholar 

  • McCloskey, L. and L. Muscatine: Production and respiration in the Red Sea coral Stylophora pistillata as a function of depth. Proc. R. Soc. (Ser. B) (in press). (1983)

  • Meeson, B. W.: Comparative physiology of a typical red tide dinoflagellate, Gonyaulax polyedra, with a cosmopolitan species, Ceratium furca, 193 pp. Ph.D. thesis, University of California, Santa Barbara 1981

    Google Scholar 

  • Meeson, B. W. and B. M. Sweeney: Adaptation of Ceratium furca and Gonyaulax polyedra (Dinophyceae) to different temperatures and irradiances: growth rates and cell volumes. J. Phycol 18, 241–245 (1982)

    Google Scholar 

  • Muscatine, L.: Productivity of zooxanthellae. In: Primary productivity in the sea, pp 381–402. Ed. by P. G. Falkowski. New York: Plenum Press (1980)

    Google Scholar 

  • Porter, I. W.: Primary productivity in the sea: reef corals in situ. In: Primary productivity in the sea, pp 403–410. Ed. by P. G. Falkowski. New York: Plenum Press 1980

    Google Scholar 

  • Prézelin, B. B.: The role of peridinin-chlorophyll a-proteins in the photosynthetic light adaptation of the marine dinoflagellate Glenodinium sp. Planta 130, 225–233 (1976)

    Google Scholar 

  • Prézelin, B. B.: Light reactions in photosynthesis. Can. Bull. Fish. aquat. Sciences 210, 1–46 (1981)

    Google Scholar 

  • Prézelin, B. B.: Photosynthetic physiology of dinoflagellates. In: The dinoflagellates, Ed. by F. J. R. Taylor. London: Blackwell Publications (in press). (1983)

    Google Scholar 

  • Prézelin, B. B. and H. A. Matlick: Nutrient-dependent low-light adaptation in the dinoflagellate Gonyaulax polyedra. Mar. Biol. 74, 141–150 (1983)

    Google Scholar 

  • Prézelin, B. B. and B. M. Sweeney: Photoadaptation of photosynthesis in Gonyaulax polyedra. Mar. Biol. 48, 27–35 (1978)

    Google Scholar 

  • Redfield, A. C., B. H. Ketcham and F. A. Richards: The influence of organisms on the composition of seawater. In: The sea, Vol. II. pp 26–77. Ed. by M. N. Hill. New York: Interscience 1963

    Google Scholar 

  • Redjalde, R.: Light adaptation strategies of hermatypic corals. Pacif. Sci. 30, p. 212 (1976)

    Google Scholar 

  • Rivkin, R. B., H. H. Seliger, E. Swift and W. H. Biggley: Lightshade adaptation by the oceanic dinoflagellates Pyrocystis noctiluca and P. fusiformis. Mar. Biol. 68, 181–191 (1982)

    Google Scholar 

  • Samuelsson, G. and K. Richardson: Photoinhibition at low quantum flux densities in a marine dinoflagellate (Amphidinium carterae). Mar. Biol. 70, 21–26 (1982)

    Google Scholar 

  • Scott, B. D. and H. R. Jitts: Photosynthesis of phytoplankton and zooxanthellae on a coral reef. Mar. Biol. 41, 307–315 (1977)

    Google Scholar 

  • Sokal, R. R. and F. J. Rohlf: Biometry. The principles and practice of statistics in biological research, 776 pp. San Francisco: W. H. & Freeman 1969

    Google Scholar 

  • Sorokin, C.: Dry weight, packed cell volume and optical density. In: Handbook of phycological methods: culture methods and growth measurements, pp 321–344. Ed. by J. R. Stein. New York: Cambridge University Press 1973

    Google Scholar 

  • Spenser-Davies, P.: Respiration in some Atlantic reef corals in relation to vertical distribution and growth form. Biol. Bull. mar. biol. Lab., Woods Hole 158, 187–194 (1980)

    Google Scholar 

  • Strickland, J. D. H. and T. R. Parsons: A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can. 167, 1–311 (1968)

    Google Scholar 

  • Svoboda, A. and T. Pormann: Oxygen production and uptake by symbiotic Aiptasia diaphana (Rapp), (Anthozoa, Coelenterata) adapted to different light intensities. In: Nutrition of the lower metazoa, pp 87–99. Ed. by D. C. Smith and Y. Tiffon. Oxford: Pergamon Press 1980

    Google Scholar 

  • Titlyanov, E. A., M. G. Shaposhnikova and V. I. Zvalinskii: Photosynthesis and adaptation of corals to irradiance. 1. Content and native state of photosynthetic pigments in symbiotic microalgae. Photosynthetica (Praha, Czechoslovakia) 14, 413–421 (1980)

    Google Scholar 

  • Trench, R. K.: The physiology and biochemistry of zooxanthellae symbiotic with marine coelenterates. I. The assimilation of photosynthetic products of zooxanthellae by two marine coelenterates. Proc. R. Soc. (Ser. B) 177, 225–235 (1971a)

    Google Scholar 

  • Trench, R. K.: The physiology and biochemistry of zooxanthellae symbiotic with marine coelenterates. II. Liberation of fixed 14C by zooxanthellae in vitro. Proc. R. Soc. (Ser. B) 177, 237–250 (1971b)

    Google Scholar 

  • Trench, R. K.: Dinoflagellates in non-parasitic symbioses. In: The dinoflagellates, Ed. by F. J. R. Taylor. London: Blackwell Publications (in press). (1983)

    Google Scholar 

  • Trench, R. K. and C. R. Fisher: Carbon dioxide fixation in Symbiodinium microadriaticum: problems with mechanisms and pathways. In: Endocytobiology. Ed. by H. E. A. Schenk and W. Schwemmler. Berlin: Walter and Gruyter & Co. (in press). (1983)

    Google Scholar 

  • Trench, R. K., D. S. Wethey and J. W. Porter: Observations on the symbiosis with zooxanthellae in the Tridacnidae (Mollusca, Bivalvia). Biol. Bull. mar. biol. Lab., Woods Hole 161, 180–198 (1981)

    Google Scholar 

  • Weiler, C. S. and R. W. Eppley: Temporal pattern of division in the dinoflagellate genus Ceratium and its application to the determination of growth rate. J. exp. mar. Biol. Ecol. 39 1–24 (1979)

    Article  Google Scholar 

  • Wethey, D. S. and J. W. Porter: Sun and shade differences in productivity of reef corals. Nature, Lond. 262, 281–282 (1976a)

    Google Scholar 

  • Wethey, D. S. and J. W. Porter: Habitat-related patterns of productivity of the foliaceous reef coral Pavona praetorta Dana. In: Coelenterate ecology and behavior, pp 59–66. Ed. by G. O. Mackie. New York: Plenum Publishing Corporation 1976b

    Google Scholar 

  • Wilkerson, F. P., G. Muller Parker and L. Muscatine: Temporal patterns of cell division in natural populations of endosymbiotic algae. Limnol. Oceanogr. 28, 1009–1014 (1983)

    Google Scholar 

  • Zar, J. H.: Biostatistical analysis, 619 pp. Englewood Cliffs, New Jersey: Prehtice Hall, Inc. 1974

    Google Scholar 

  • Zvalinskii, V. I., V. A. Leletkin, E. A. Titlyanov and M. G. Shaposhnikova: Photosynthesis and adaptations of corals to irradiance. 2. Oxygen exchange. Photosynthetica (Praha, Czechoslovakia) 14, 422–430 (1980)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, University of California at Santa Barbara, 93106, Santa Barbara, California, USA

    S. S. Chang, B. B. Prézelin & R. K. Trench

  2. the Marine Science Institute, University of California at Santa Barbara, 93106, Santa Barbara, California, USA

    S. S. Chang, B. B. Prézelin & R. K. Trench

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  1. S. S. Chang
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  2. B. B. Prézelin
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  3. R. K. Trench
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Communicated by N. D. Holland, La Jolla

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Chang, S.S., Prézelin, B.B. & Trench, R.K. Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum . Marine Biology 76, 219–229 (1983). https://doi.org/10.1007/BF00393021

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