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Immobilization of the enzyme L-asparaginase from E. coli on polysaccharides I. Covalent binding with insoluble Sepharoses and CM-cellulose

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Summary

Immobilized L-asparaginase has been synthesized by various methods of covalent binding to insoluble Sepharoses and CM-cellulose.

Some kinetic properties of the preparations obtained, the dependence of the activity on the pH of the medium, and their stability have been investigated.

It has been established that the immobilized L-asparaginase possesses increased heat resistance, stability on storage, and stability to competing inhibitors.

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Authors
  1. A. S. Karsakevich
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  2. R. A. Zhagat
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  3. V. Yu. Son
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Additional information

Institute of Organic Synthesis, Academy of Sciences of the Latvian SSR, Riga. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 639–643, September–October, 1976.

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Karsakevich, A.S., Zhagat, R.A. & Son, V.Y. Immobilization of the enzyme L-asparaginase from E. coli on polysaccharides I. Covalent binding with insoluble Sepharoses and CM-cellulose. Chem Nat Compd 12, 571–574 (1976). https://doi.org/10.1007/BF00565189

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  • DOI: https://doi.org/10.1007/BF00565189

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