Abstract
In the following, we describe the application of the DIG/antiDIG system for the (structural) analysis of glycoproteins on blots. Special emphasis is being placed on the variety of the different DIG applications in order to obtain structural information concerning the glycoprotein carbohydrate chains. Prominent among them is the use of lectins with well-known specificities for carbohydrate structures.
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Haselbeck, A. and Hösel, W. (1990),Glycoconjugate J. 7, 63–74.
Haselbeck, A., Schickaneder, E., von der Eltz, H., and, Hösel, W. (1990),Anal. Biochem. 191, 25–30.
Maley, F., Trimble, R. B., Tarentino, A. L., and Plummer, T. H., Jr. (1989),Anal. Biochem. 180, 195–204.
Tarentino, A. L., Trimble, R. B., and Plummer, T. H., Jr. (1989),Methods Cell Biol. 32, 111–139.
Kniep, B. and Mühlradt, P. F. (1990),Anal. Biochem. 188, 5–8.
Sata, T., Zuber, J., and Roth, J. (1990),Histochemistry 94, 1–11.
Braun, W. and Abraham, R. (1989),Electrophoresis 10, 249–253.
Schipper, H. J., Kruse, H., and Reiber, H. (1984),Sci. Tools 31, 5,6.
Bowen, B., Steinberg, J., Laemmli, U. K., and Weintraub, H. (1980),Nucleic Acids Res. 8, 1–3.
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This article first appeared in: Hounsell, E. F., ed. (1993),Methods in Molecular Biollogy, vol. 14: Glycoprotein Analysis in Biomedicine, Humana Press Inc., Totowa, NJ.
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Haselbeck, A., Hösel, W. Immunological detection of glycoproteins on blots based on labeling with digoxigenin. Appl Biochem Biotechnol 42, 207–219 (1993). https://doi.org/10.1007/BF02788054
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DOI: https://doi.org/10.1007/BF02788054