Abstract
Fusarium semitectum, which occurs frequently among the fungal microflora associated with seedling disease of cotton, was examined using traditional mycological characteristics and molecular methods. The 29 Fusarium semitectum isolates from different cotton-producing areas and different host genotypes in Egypt could be divided undoubtedly into five groups based on cultural characteristics or Dna fingerprinting methods. The isolates showed a high level of variability in aerial mycelia growth, mycelia colour and radial mycelia growth (colony diameter) on potato dextrose agar. The intra-specific genomic relatedness of F. semitectum isolates was detected by Aflp and Rapd-Pcr fingerprinting methods and no particular difference was found between the results with the two fingerprint techniques.
Five genomic groups were obtained with both Rapd-Pcr and Aflp techniques. A good correlation of the five genomics groups and cultural characteristics were found, but no correlation between the results of the genetic analysis with the host genotype and geographic origin could be observed. The results indicate the utility of Rapd-Pcr and Aflp for identifying and monitoring intra-specific genetic variability for F. semitectum isolates.
Zusammenfassung
Fusarium semitectum, ein Pathogen, das häufig mit anderen Sämlingskrankheiten assoziiert ist, wurde mit traditionellen mykologischen und molekularen Techniken untersucht. Die 29 Isolate, die aus unterschiedlichen Anbauregionen der Baumwolle und Wirtsgenotypen isoliert wurden, konnten aufgrund ihrer morphologischen Charakteristika und der Ergebnisse aus den Fingerprints eindeutig in fünf Gruppen aufgeteilt werden. Die Isolate zeigten eine hohe Variabilität im Luftmycelwachstum, in der Pigmentierung und im radialem Myzelwachstum auf Pd-Agar.
Die intraspezifische, genomische Verwandtschaft der Isolate konnte mit der Aflp- und Rapd-Pcr Fingerprint-Methode nachgewiesen werden, wobei keine wesentlichen Unterschiede in den Ergebnissen der beiden Fingerprint-Techniken entdeckt wurden. Es konnten ebenfalls fünf genomische Gruppen abgeleitet werden. Allerdings konnte keine Korrelation zwischen den Ergebnissen der Fingerprint-Analyse und dem Wirtsgenotyp bzw. der Herkunft der Isolate hergestellt werden.
Die Ergebnisse zeigen, dass mit der Rapd-Pcr und Aflp-Technik eine Klassifizierung von F. semitectum möglich ist und die intraspezifische, genetische Variabilität bestimmt werden kann.
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Literature
Appel, D. J., T. R. Gordon: Local and regional variation in populations of Fusarium oxysporum from agricultural field soils. — Phytopathology 84, 786–791, 1994.
Asstgbetse, K. B., D. Fernandez, M. P. Doubtos, J. P. Getger: Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random ampilified polymorphic Dna (Rapd) analysis. — Phytopathology 84, 622–626, 1994.
Booth, C., B. C. Sutton: Fusarium pallidoroseum, the correct name for F. semitectum Auct. — Trans. Brit. Mycol. Soc. 83, 702–704, 1984.
Brown, J. K. M.: The choice of molecular marker methods for population genetic studies of plant pathogens. — New Phytol. 133, 183–195, 1996.
Chen-Shang, W., M. Hutqtn, W. Hut, S. W. Chen, M. Hq, H. Wang: Simultaneous identification of melon postharvest decay pathogens by the random amplified polymorphic Dna analysis with polymerase chain reaction. — Res. Prog. in Plant Prot. and Plant Nutr. 57, 109–114, 1999.
Creste, S., A. Tulmann-Neto, A. Ftguetra: Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. — Pl. Mol. Biol. Rep. 19, 1–8, 2001.
Donaldson, G. C., L. A. Ball, P. E. Axlrood, N. L. Glass: Primer sets developed to amplify conserved genes from filamentous ascomycetes are useful in differentiating Fusarium species associated with conifers. — Theor. Appl. Gen. 61, 1331–1340, 1995.
El-Samawaty, A. M. A.: Studies on cotton root rot disease. — MSc. Thesis, Univ. Assiut, Egypt, 1999.
Ellworth, D. L., K. D. Rittenhouse, R. L. Honeycut: Artifactual variation in randomly amplified polymorphic DNA banding patterns. — Biotechniques 14, 214–217, 1993.
Feng, J. I. E., S. Wen, S. Leiyan, M. A. Cun, J. Feng, W. J. Sun, C. L. Y. Shi: RAPD analysis of physiologic races of Fusarium oxysporum f. sp. vasinfectum in China. — Mycosystema 19, 45–50, 2000.
Gerlach W., H. Nirenberg: The genus Fusarium: A pictorial atlas. — Mitt. Biol. BundAnst. Ld. u. Forstwirtsch., Berlin 209, 1–406, 19&
Gonzalez, M. M., E. Z. Rodriguez, J. L. Jacabo, F. Hernandez, J. Acosta, O. Martinez J. Simpson: Characterization of Mexican isolates of Colletotrichum lindemuthianum by using differential cultivars and molecular markers. — Phytopathology 88, 292–299, 1998.
Gordon, T. R., D. Okamoto: Variation within and between populations of Fusarium oxysporum based on vegetative compatibility and mitochondrial DNA. — Can. J. Bot. 70, 1211–1217, 1992.
Hillocks, R. J.: Cotton diseases. — CAB. International, Wallingford, United Kingdom, 1992.
Janssen, P., R. Coopman, G. Huys, J. Swings, H. Bleeker, P. Vos, M. Zabeau, K. Kersters: Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. — Microbiol. 142, 1881–1893, 1996.
Kiprop, E. K., J. P. Baudoin, A. W. Mwang’Ombe, P. M. Kimani, G. Mergeai, A. Maquet: Characterization of Kenyan isolates of Fusarium udum from pigeonpea [Cajanus cajan (L.) Millsp.] by cultural characteristics, aggressiveness and AFLP analysis. — J. Phytopath. 150, 517–527, 2002.
Leissner, C. E. W., M. L. Niessen, R. F. Vogel: Use of the AFLP technique for the identification and discrimination of Fusarium graminearum. — Cereal Res. Commun. 25, 555–556, 1997.
Majer, D., R. Mithen, B. Lewis, P. Vos, R. P. Oliver: The use of AFLP fingerprinting for the detection of genetic variation in fungi. — Mycol. Res. 100, 1107–1111, 1996.
Mayek-Perez, N., Z. Lopez-Castaneda, M. Gonzalez-Chavira, R. Garcia-Espinosa, J. Acosta-Gallegos, O. Martinez-Dela-Vega, J. Simpson: Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. — Physiol. Mol. Pl. Pathol. 59, 257–264.
Mantel, N.: The detection of disease clustering and a generalized regression approach. — Cancer Res. 27, 209–220, 1967.
Miller, S. A.: Detecting propagules of plant pathogenic fungi. — Adv. Bot. Res. 23, 73–102, 1996.
Nei, M., W. H. Li: Mathematical model for studying genetic variation in terms of restriction endo-nucleases. — Proc. Natl. Acad. Sci. USA, 76, 5269–5273, 1979.
O’Neill, N. R., B. A. Bailey, P. B. Van-Berkum: AFLP, a novel PCR-based DNA analysis technique, reveals level of genomic variation within species of Colletotrichum, Fusarium and Dendryphion. — In: 7th International Congress of Plant Pathology, Edinburgh, Scotland, 9–16 August, 1998.
Palmateer, A., K. McLean, G. Morgan-Jones: Fungi involved in the seedling complex on Albama cotton. — Mag. Res. Ala. Agric. Exp. Stn. 48, 3, 2001.
Pizzinatto, M. A., J. O. M. Menten: Pathogenicity of eight Fusarium spp. isolated from seeds to cotton seedlings. — Summa Phytopathol. 17, 124–134, 1991.
Rayner, R. W.: A mycological colour chart. — Commonwealth Mycological Institute, Kew, UK, 1970.
Roux, K. H.: Optimization and troubleshooting in PCR. — PCR Methods Appl. 4, 5185–5194, 1995.
Sambrock, J., Fritsch, E. F. & T. Maniatis: Molecular cloning: A laboratory manual (sec. Edition). Cold Spring Harbour Press, New York, U. S. A., 1989.
Schnieder, F., G. Koch, C. Jung, J.-A. Verreet: Genotypic diversity of the wheat leaf blotch pathogen Mycosphaerella gramincola (anamorph Septoria tritici) in Germany. — Eur. J. Pl. Pathol. 107, 285–290, 2001.
Smith, S. N., J. E. Devay, H. W. Hsui, L.-H. Jen, W. H. Hsieh, H. J. Lee: Soil-borne populations of Fusarium oxysporum f. sp. vasinfectum, a cotton wilt fungus in California fields. — Mycologia 93, 737–743, 2001.
Sneath, P. H. A., R. R. Sokal: Numerical taxonomy. — Freeman and Company, San Francisco, 1973.
Soleimany, M. J., G. A. Hedjaroude, J. Zad: Studies on pathogenicity of some seedborne Fusarium species on cotton seedling.— Iran J. Pl. Pathol. 29, 19–20, 1993.
Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van der Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, M. Zabeau: Aflp: a new technique for Dna fingerprinting. — Nucleic Acids Res. 23, 4407–4424, 1995.
Wang, G. C., Z. F. Gu, X. Lou, Q. M. Ye: Studies on the pathogens of Fusarium red rot of cotton. — Acta Phytopathol. Sin. 22, 211–215, 1992.
Welsh, J., M. McClelland: Fingerprinting genomes using Pcr with arbitrary primers. — Nucleic Acids Res. 18, 7213–7218, 1990.
Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, S. V. Tingey: Dna polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res. 18, 6531–6535, 1990.
Yli-Mattila, T., S. Paavanen, A. Hannukkala, P. Parikka, R. Tahvonen, R. Karjalainen: Iso- zyme and Rapd-Pcr analysis of Fusarium avenaceum strains from Finland. — Pl. Pathol. 45, 126–134, 1996.
Zabeau, M., P. Vos: Selective restriction fragment amplification: a general method for Dna fingerprinting. — European Patent Office, publication 0 534 858 A1, bulletin 93/13, 1993.
Zhang, J. X., C. R. Howell, J. L. Starr, M. H. Wheeler, J. X. Zhang: Frequency of isolation and the pathogenicity of Fusarium species associated with roots of healthy cotton seedlings. — Mycol. Res. 100, 747–752, 1996.
Zhu, H., F. Qu, L. H. Zhu: Isolation of genomic Dnas from plants, fungi and bacteria using benzyl chloride. — Nucleic Acids Res. 2, 5279–5280, 1993.
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Abd-Elsalam, K.A., Schnieder, F., Asran-Amal, A. et al. Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics. J Plant Dis Prot 110, 409–418 (2003). https://doi.org/10.1007/BF03356118
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DOI: https://doi.org/10.1007/BF03356118