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Induction of systemic resistance in faba bean (Vicia faba L.) to bean yellow mosaic potyvirus (BYMV) via seed bacterization with plant growth promoting rhizobacteria

Induzierte systemische Resistenz gegenüber dem Bohnengelbmosaikvirus in der Dicken Bohne (Vicia faba L.) durch Saatgutbehandlung mit wachstumsfördernden Rhizobakterien

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Abstract

In a greenhouse experiment, two plant growth promoting rhizobacteria „Pgpr“ strains (Pseudomonas fluorescens FB11 and a Rhizobium leguminosarum bv. viceae FBG05) isolated from roots of faba bean plants were tested singly and in combination as seed inoculants for induction of systemic resistance in faba bean against bean yellow mosaic potyvirus (BYMV). The results demonstrated that BYMV challenged plants emerged from Pseudomonas inoculated seeds not only showed a pronounced and significant reduction in percent disease incidence (PDI) but also a significant reduction in virus concentration (ELISA) in the challenged plants, compared to the nonbacterized, challenged plants. Rhizobium singly also showed a significant reduction in both Pdi and ELISA value, but the reduction was less pronounced than that resulting from Pseudomonas inoculation. Combined inoculation with Pseudomonas and Rhizobium showed no additional significant reduction in Pdi or ELISA value compared with Pseudomonas singly. Appreciable and significant increase in both salicylic acid level and peroxidase activity was observed in leaves of all Pgpr inoculated plants compared to other treatments. Since the Pgpr inoculants (Pseudomonas and Rhizobium) and the pathogen (BYMV) remained spatially separated, it can be concluded that the tested Pseudomonas or Rhizobium strains induced systemic resistance in faba bean against BYMV.

Zusammenfassung

Zwei das Pflanzenwachstum fördernde Rhizobakterienisolate (Pgpr) aus Wurzeln der Dicken Bohne, Pseudomonas fluorescens Fb11 and Rhizobium leguminosarum bv. viceae Fbg05, wurden einzeln und kombiniert als Saatgutbehandlungsmittel hinsichtlich einer induzierten Resistenz der Dicken Bohne gegenüber dem Bohnengelbmosaikvirus (BYMV) im Gewächshaus überprüft. Dem BYMV ausgesetzte Bohnenpflanzen zeigten nach einer Pseudomonas-Behandlung im Vergleich zur unbehandelten Kontrolle nicht nur eine deutlich verminderte Befallshäufigkeit im Bestand (Bhb), sondern darüber hinaus eine signifikant verminderte Viruskonzentration im ELISA-Test. Eine Rhizobium-Behandlung des Saatguts bewirkte ebenfalls signifikant verminderte BHB- und ELISA-werte, die allerdings unter denen Pseudomonas-behandelter Pflanzen lagen. Eine kombinierte Saatgutbehandlung mit Pseudomonas und Rhizobium übertraf die Wirkung der Pseudomonas-Einzelbehandlung nicht. Die Blätter aller PGPR-behandelten Pflanzen zeigten gegenüber der unbehandelten Kontrolle erhöhte Salicylsäurekonzentrationen und Peroxidaseaktivitäten Die räumliche Trennung der als Saatgutbehandlungsmittel verwendeten Pseudomonas- und Rhizobium- Isolate vom Pathogen (BYMV) deutet darauf hin, dass sie in den Bohnenpflanzen eine induzierte Resistenzreaktion gegenüber dem Virus auslösten.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum Branch, Cairo University, Egypt

    M. Elbadry & H. Gamal-Eldin

  2. Department of Botany, Faculty of Science, Fayoum Branch, Cairo University, Egypt

    R. M. Taha

  3. Department of Agricultural Microbiology, Virology Lab. Faculty of Agriculture, Ain Shams University, Egypt

    K. A. Eldougdoug

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  1. M. Elbadry
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  2. R. M. Taha
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  3. K. A. Eldougdoug
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  4. H. Gamal-Eldin
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Correspondence to M. Elbadry.

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Elbadry, M., Taha, R.M., Eldougdoug, K.A. et al. Induction of systemic resistance in faba bean (Vicia faba L.) to bean yellow mosaic potyvirus (BYMV) via seed bacterization with plant growth promoting rhizobacteria. J Plant Dis Prot 113, 247–251 (2006). https://doi.org/10.1007/BF03356189

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