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GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon

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Abstract

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Map-based cloning identified CmGL that encodes a HD-ZIP type IV transcription factor that controls multicellular trichome initiation in melon.

Abstract

Trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. The regulatory pathway of unicellular trichomes has been well studied in the model plant Arabidopsis. Little is known about the genetic control and regulation of trichome development in melon (Cucumis melo L.) which has multicellular trichomes. In this study, we identified a melon mutant, cmgl, which showed completely glabrous on all aerial organs. A bulked segregant analysis was conducted to identify polymorphic markers for linkage analysis in a population with 256 F2 plants, which allowed to locate the cmgl locus in melon chromosome VIII. Next-generation sequencing-aided marker discovery and fine mapping in a large population with 1536 F2 plants narrowed the candidate gene region to 12 kb that harbored only one candidate gene for cmgl, which encoded a class IV homeodomain-associated leucine zipper transcription factor. Four SNPs in the coding region of the CmGL gene were identified between the two parental lines; a single base substitution from C to A resulted in a premature termination codon and a truncated protein in the cmgl. The SNP was converted into a dCAPS marker, which showed co-segregation in the F2 population and 564 melon accessions. Result of this study will be helpful for better understanding of genetic control of trichome development in melon and marker-assisted selection in developing new cultivars.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No. 31601776), the Scientific Innovation Fund of Henan Agricultural University (KJCX2016A15), and the International Scientific and Technological Cooperation Project of Henan Province.

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Author notes

  1. Huayu Zhu and Xiaofen Sun contributed equally to this work.

Authors and Affiliations

  1. College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China

    Huayu Zhu, Xiaofen Sun, Pengyao Song, Qianmei Hu, Xiaojing Zhang, Xiang Li, Jianbin Hu, Shouru Sun & Luming Yang

  2. School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China

    Qi Zhang & Junsong Pan

  3. USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI, 53706, USA

    Yiqun Weng

  4. Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou, 450002, China

    Jianbin Hu & Shouru Sun

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  1. Huayu Zhu
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  2. Xiaofen Sun
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Corresponding author

Correspondence to Luming Yang.

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The experiments comply with the current laws of the country in which we were performed.

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The authors declare that they have no conflict of interest.

Accession codes

The genomic resequence datasets of two melon materials M465 and NSL73046 have been deposited into the NCBI sequence read archive (SRA) SRP119828 under accessions SRS2585930 and SRS2585931.

Additional information

Communicated by Herman J. van Eck.

Electronic supplementary material

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122_2017_3019_MOESM1_ESM.pdf

Figure S1 Phylogenetic tree of CmGL and HD-ZIP IV proteins in other species. The HD-ZIP IV proteins used for phylogenetic analysis including 16 from Arabidopsis (ATML-1, PDF2, ANL2, GL2, HDG1 to HDG12), 1 from tomato (Wo), 4 from maize (ZmOCL1–ZmOCL4), 2 from rice (OsRoc1, OsRoc5), and 6 from cotton (GhHD-1A, GhHD-1D, GaHOX1, GaHOX2, GaHOX3-A and GaHOX3-D) (PDF 15 kb)

Figure S2 Phylogenetic tree of HD-ZIP IV genes from cucumber and melon (PDF 8 kb)

122_2017_3019_MOESM3_ESM.xlsx

Table S1 The list of melon germplasm used for analyzing allelic diversity of CmGL gene. Table S2 The list of primers used for mapping and qRT-PCR analysis (XLSX 33 kb)

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Zhu, H., Sun, X., Zhang, Q. et al. GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon. Theor Appl Genet 131, 569–579 (2018). https://doi.org/10.1007/s00122-017-3019-9

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