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Characterisation of barley resistance to rhynchosporium on chromosome 6HS

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Abstract

Key message

Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley.

Abstract

Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

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Acknowledgements

This work was funded by the Scottish Food Security Alliance (SFSA) and the Bavarian State Ministry of Food, Agriculture and Forestry. MB, LR, RW, MEL and AA were supported by the Scottish Government Rural and Environment Science and Analytical Services (RESAS). AA and MB were also funded by the BBSRC-CIRC Project BB/J019569/1. KH was supported by the Federal Office of Agriculture and Food (BLE) under Grant No 28-1-41.009-06.

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  1. The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK

    Max Coulter, Micha Bayer, Luke Ramsay, Robbie Waugh, Mark E. Looseley & Anna Avrova

  2. Institute for Crop Science and Plant Breeding, Bavarian State Research Center for Agriculture, Am Gereuth 2, 85354, Freising, Germany

    Bianca Büttner, Kerstin Hofmann & Günther Schweizer

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  1. Max Coulter
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Coulter, M., Büttner, B., Hofmann, K. et al. Characterisation of barley resistance to rhynchosporium on chromosome 6HS. Theor Appl Genet 132, 1089–1107 (2019). https://doi.org/10.1007/s00122-018-3262-8

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