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Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Stomatal Closure in Arabidopsis

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Abstract

Cysteine desulfhydrase (CDes) can catalyze the degradation of cysteine producing hydrogen sulfide. In this study, d-cysteine desulfhydrase from wheat (TaD-CDes) was cloned and overexpressed in Arabidopsis thaliana. The physiological effects of TaD-CDes were determined by investigating seed germination, root growth, stomatal closure, and drought resistance in the TaD-CDes plants. Results showed that, compared with wild-type plants (WT), seed germination, root growth, and stomatal closure of the TaD-CDes plants were more sensitive to ABA, resulting from up-regulation of ABA-responsive genes (such as PYR1, ABI1, ABI2, HAB1, HAB2, SnRK2, ABF2, and ABF4). Moreover, although TaD-CDes mediated ABA-induced stomatal closure, TaD-CDes-overexpressing plants did not show higher drought resistance than WT, which might be attributed to their increased stomatal densities.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31501238), the National Key Research and Development Program of China (2017YFD0300408), Scientific Research Foundation of the Higher Education Institutions of He’nan Province, China (15A180040), and Science and Technology Innovation Fund of Henan Agricultural University (KJCX2015A13).

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Authors and Affiliations

  1. College of Life Science, Henan Agricultural University, Zhengzhou, 450002, China

    Hua Li, Yang Zhang, Xiao Li, Ruili Xue & Huijie Zhao

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  1. Hua Li
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  2. Yang Zhang
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  3. Xiao Li
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  4. Ruili Xue
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  5. Huijie Zhao
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Correspondence to Hua Li.

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Li, H., Zhang, Y., Li, X. et al. Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Stomatal Closure in Arabidopsis. J Plant Growth Regul 37, 1175–1184 (2018). https://doi.org/10.1007/s00344-018-9817-8

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  • DOI: https://doi.org/10.1007/s00344-018-9817-8

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