Abstract
This study aims to evaluate the prevalence of trichomonads in the subgingival biofilm of patients with periodontitis. Secondarily, the trichomonad presence was related to patient characteristics and periodontal clinical parameters, in order to highlight the factor favoring the development of these protozoans. Subgingival biofilm samples were collected from at least two diseased and one healthy site in 50 patients suffering from periodontitis. Trichomonads were identified using phase contrast microscopy. All patient characteristics and periodontal clinical parameter data were then statistically analyzed. From the 50 patients examined, 195 sites were sampled, including 145 diseased ones. Trichomonads were only observed on 16 of the 145 diseased sites (11%) and none in the other 50 healthy sites. Based on these results, 20% (n = 10) of patients were positive for the presence of trichomonads from at least one of the diseased sites collected. Tooth mobility, substantial supra-gingival dental deposits, and severe clinical attachment loss were statistically associated with trichomonad presence. If the subgingival biofilm of male patients over the age of 50 seemed to be more frequently contaminated with trichomonads, this data was not statistically supported. This preliminary study indicates for the first time that in periodontitis-involved patients, trichomonads are observed in the subgingival biofilm collected from diseased sites with severe bone loss, but not from healthy teeth. Further investigations are needed to fully explore the role of this microorganism in the etiology of periodontal disease.
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References
Amano A (2010) Host-parasite interactions in periodontitis: microbial pathogenicity and innate immunity. Periodontol 2000(54):9–14
Armitage GC (1999) Development of a classification system for periodontal diseases and conditions. Northwest Dent 4:1–6
Avila M, Ojcius DM, Yilmaz O (2009) The oral microbiota: living with a permanent guest. DNA Cell Biol 28:405–411. https://doi.org/10.1089/dna.2009.0874
Bózner P, Demes P (1991) Degradation of collagen types I, III, IV and V by extracellular proteinases of an oral flagellate Trichomonas Tenax. Arch Oral Biol 36:765–770
Cambon M, Petary AF, Guillot J, Glander I, Deguillaume J, Coulet M (1979) A study of the frequency of protozoa and yeasts isolated from the parodontium of 509 subjects. Pathol Biol (Paris) 10:603–606
Crucitti T, Jespers V, Mulenga C, Khondowe S, Vandepitte J, Buvé A (2010) Trichomonas vaginalis is highly prevalent in adolescent girls, pregnant women, and commercial sex workers in Natola, Zambia. Sex Transm Dis 4:223–227. https://doi.org/10.1097/OLQ.0b013e3181c21f93
El Sibaei MM, Abdel-Fattah NS, Ahmed SA, Abou-Seri HM (2012) Growth kinetics, antigen profiling, and proteinase activity of Egyptian Trichomonas tenax isolates derived from patients having oral infections. Exp Parasitol 130:416–422. https://doi.org/10.1016/j.exppara.2012.01.018
Feki A, Molet B, Haag R, Kremer M (1981) Protozoa of the human oral cavity (epidemiological correlations and pathogenic possibilities). J Biol Buccale 9:155–161
Ferrara A, Conca R, Grassi L Jr, De Carneri I (1986) Possible pathogenic role of Trichomonas tenax in chronic periodontitis. Ann Ist Super Sanita 221:253–255
Franjola R, Puga S, Matamala F (1978) Preliminary investigation in Trichomonas tenax in the city of Valvidia, Chile. Bol Chil Parasitol 33:37–38
Gerbod D, Edgcomb VP, Noël C, Vanácová S, Wintjens R, Tachezy J, Sogin ML, Viscogliosi E (2001) Phylogenetic relationships of class II fumarase genes from trichomonad species. Mol Biol Evol 18(8):1574–1584. https://doi.org/10.1093/oxfordjournals.molbev.a003944
Ghabanchi J, Zibaei M, Daguighafkar M, Sarbazie AH (2010) Prevalence of oral Entamoeba gingivalis and Trichomonas tenax in patients with periodontology disease and healthy population in Shiraz, Southern Iran. Indian J Dent Res 21:89–91. https://doi.org/10.4103/0970-9290.62821
Greene JC, Vermillion JR (1964) The simplified oral hygiene index. J Am Dent Assoc 68:7–13
Hersh SM (1985) Pulmonary trichomoniasis and Trichomonas tenax. J Med Microbiol 20:1–10
Jakobsen EB, Friis-Moller EB, Friis EB (1987) Trichomonas species in subhepatic abscess. Eur J Clin Microbiol 6:296–297
Lange DE, Stockmann H, Höcker K (1983) Presence and identification of protozoa in the human oral cavity. Dtsch Zahnärz HZ 38:906–910
Linke HA, Gannon JT, Obin JN (1989) Clinical survey of Entamoeba gingivalis by multiple sampling in patients with advanced periodontal disease. Int J Parasitol 19:803–808
Loë H, Silness J (1963) Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 21:533–551
Lopez-Escamilla E, Sanchez-Aguillon F, Alatorre-Fernandez CP, Aguilar-Zapata D, Arroyo-Escalante S, Arellano T, Mancada-Barron D, Romero-Valdovinos M, Martinez-Hernandez F, Rodriguez-Zulueta P, Maravilla P (2013) New Tetratrichomonas species in two patients with pleural empyema. J Clin Microbiol 51:3143–3146. https://doi.org/10.1128/JCM.01056-13
Lyons T, Scholten T, Palmer JC, Stanfield E (1983) Oral amoebiasis: the role of Entamoeba gingivalis in periodontal disease. Quintessence Int Dent Dig 1412:1245–1248
Madhi NK, Al-Saeed AT (1993) Trichomonas tenax in Basrah, Iraq. J Pak Med Assoc 43:261–262
Meloni D, Mantini C, Goustille J, Desoubeaux G, Maakaroun-Vermesse Z, Chandenier J, Gantois N, Duboucher C, Fiori PL, Dei-Cas E, Duong TH, Viscogliosi E (2011) Molecular identification of Pentatrichomonas hominis in two patients with gastrointestinal symptoms. J Clin Pathol 64:933–935. https://doi.org/10.1136/jcp.2011.089326
Miligi G, Magaudda Borzi L, Mento G (1964) The incidence of carriers of Trichomonas tenax and of Entamoeba gingivalis in some zones of the province of Messina. Arch Ital Sci Med Trop 45:95–99
Nagao E, Yamamoto A, Igarashi T, Goto N, Sasa R (2000) Two distinct hemolysins in Trichomonas tenax ATCC 30207. Oral Microbiol Immunol 15:355–359
Perez-Jaffe L, Katz R, Gupta PK (1998) Entamoeba gingivalis identified in a left upper neck nodule by fine-needle aspiration: a case report. Diagn Cytopathol 18:458–461
Ribeiro LC, Santos C, Benchimol M (2015) Is Trichomonas tenax a parasite or a commensal? Protist 166:196–210. https://doi.org/10.1016/j.protis.2015.02.002
Rivera F, Ramírez P, Vilaclara G, Robles E, Medina F (1983) A survey of pathogenic and free-living amoebae inhabiting swimming pool water in Mexico City. Environ Res 32:205–211
Rousset JJ, Laissus B, Michel C (1970) Preliminary data on canine enzootics due to Trichomonas canistomae and Entamoeba gingivalis in Parisian dogs. CR Seances Soc Biol Fil 164:2468246–2468249
Segovic S, Buntak-Kobler D, Galic N, Katunaric M (1998) Trichomonas tenax proteolytic activity. Coll Antropol 22:45–49
Silness J, Loë H (1964) Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 22:121–135
Wantland WW, Lauer D (1970) Correlation of some oral hygiene variables with age, sex, and incidence of oral protozoa. J Dent Res 49:293–297
Yamamoto A, Asaga E, Nagao E, Igorashi T, Gato N (2000) Characterization of the cathepsin B-like proteinase of Trichomonas tenax ATCC (30207). Oral Microbial Immunol 15:360–364
Acknowledgements
We would like to thank Brice KABONGO for his help in the examination of patient subgingival biofilms, Louise BOUTELLIEZ for her assistance in formatting the article, and Marie-Pascale HIPPOLYTE for her English language expertise.
Funding
This study was supported by a grant from the Conseil Regional du Grand Nancy and from the Colgate Palmolive Company.
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After approval of this study by the NUH Ethics Committee, this clinical trial was then submitted to the clinicaltrials.com website and received the following number: NCT03178838. Patients received an information note for this study and have given permission for the use of their personal clinical and microbiological data.
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Video of sub-gingival biofilm with trichomonad (Phase contrast microscopy, X100). (MP4 179,061 kb)
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Bisson, C., Lec, PH., Blique, M. et al. Presence of trichomonads in subgingival biofilm of patients with periodontitis: preliminary results. Parasitol Res 117, 3767–3774 (2018). https://doi.org/10.1007/s00436-018-6077-2
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DOI: https://doi.org/10.1007/s00436-018-6077-2