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Identification and characterization of a new potyvirus infecting cucurbits

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Abstract

A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.

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Acknowledgements

This study was carried out at IPAVE-CIAP-INTA and was partially supported by INTA and CONICET. Dr. Luis Conci kindly provided positive samples.

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Authors and Affiliations

  1. Instituto de Patología Vegetal (IPAVE-CIAP-INTA), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina

    M. C. Perotto & V. C. Conci

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina

    M. C. Perotto, E. A. Pozzi, M. G. Celli, C. E. Luciani & V. C. Conci

  3. Estación Experimental Agropecuaria San Pedro (EEA San Pedro-INTA), Ruta 9, km 170, 2930, San Pedro, Buenos Aires, Argentina

    M. S. Mitidieri

Authors
  1. M. C. Perotto
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  2. E. A. Pozzi
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  3. M. G. Celli
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  4. C. E. Luciani
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  5. M. S. Mitidieri
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  6. V. C. Conci
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Corresponding author

Correspondence to M. C. Perotto.

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Funding

This study was funded by CONICET (PIP no. 112-20110111016) and INTA (PNHFA 1106072, PNPV 1135024).

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All authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Stephen John Wylie.

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Perotto, M.C., Pozzi, E.A., Celli, M.G. et al. Identification and characterization of a new potyvirus infecting cucurbits. Arch Virol 163, 719–724 (2018). https://doi.org/10.1007/s00705-017-3660-2

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  • DOI: https://doi.org/10.1007/s00705-017-3660-2

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