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Copper microlocalisation and changes in leaf morphology, chloroplast ultrastructure and antioxidative response in white lupin and soybean grown in copper excess

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Abstract

The microlocalisation of Cu was examined in the leaves of white lupin and soybean grown hydroponically in the presence of 1.6 (control) or 192 μM (excess) Cu, along with its effect on leaf morphology, (ultra)structure and the antioxidative response. The 192 μM dose led to a reduction in the total leaf area and leaf thickness in both species, although more strongly so in white lupin. In the latter species it was also associated with smaller spongy parenchyma cells, and smaller spaces between them, while in the soybean it more strongly reduced the size of the palisade parenchyma and epidermal cells. Energy-dispersive X-ray microanalysis showed that under Cu excess the metal was mainly localised inside the spongy parenchyma cells of the white lupin leaves, and in the lower epidermis cell walls in those of the soybean. Cu excess also promoted ultrastructural chloroplast alterations, reducing the photosynthetic capacity index and the green area of the leaves, especially in the soybean. Despite this, soybean appeared to be more tolerant to Cu excess than white lupin, because soybean displayed (1) lower accumulation of Cu in the leaves, (2) enhanced microlocalisation of Cu in the cell walls and (3) greater levels of induced total –SH content and superoxide dismutase and catalase activities that are expected for better antioxidative responses.

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Acknowledgments

Funding for this study was provided by the Spanish MCyT (project CTM2010-21922-C02-02/TECNO), the Autonomous Community of Madrid (project S2009/AMB-1478) and the Junta de Comunidades de Castilla-La Mancha (project POII10-0211-5015). The soybean seeds and Bradyrhizobium japonicum strain were a kind gift of Dr. F. Temprano (IFAPA, Junta de Andalucía). The white lupin seeds were a gift of Dr. A. Gil Aragón (Centro de Investigación Finca La Orden-Valdesequera, Junta de Extremadura). We thank F. Pinto (ICA-CSIC) for expert assistance with LTSEM-EDXMA, S. Fajardo and C. Morcillo (ICA-CSIC) for technical assistance with the microscopy procedures, and Adrian Burton for linguistic assistance.

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Authors and Affiliations

  1. Dpto. Química Agrícola, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Beatriz Sánchez-Pardo & Pilar Zornoza

  2. Dpto. Protección Vegetal, Instituto de Ciencias Agrarias, CSIC, Serrano 115 dpdo, 28006, Madrid, Spain

    Mercedes Fernández-Pascual

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  1. Beatriz Sánchez-Pardo
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  2. Mercedes Fernández-Pascual
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  3. Pilar Zornoza
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Correspondence to Pilar Zornoza.

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Sánchez-Pardo, B., Fernández-Pascual, M. & Zornoza, P. Copper microlocalisation and changes in leaf morphology, chloroplast ultrastructure and antioxidative response in white lupin and soybean grown in copper excess. J Plant Res 127, 119–129 (2014). https://doi.org/10.1007/s10265-013-0583-1

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