An original method for preparing injection replicas of blood vessels for studies of the microcirculatory bed of regenerated bone using scanning electron microscopy in experiments on dogs undergoing surgical limb lengthening is described. The results provide evidence of active angiogenesis processes.
Similar content being viewed by others
References
Vlasov, A. I., Elsukov, K. A., and Kosolapov, I. A., Electron Microscopy. A Textbook [in Russian], Bauman Moscow State Technical University, Moscow (2011).
Prokop’ev, Yu. A., Fomenko, D. V., Kizichenko, N. V., Kudryavtseva, Yu. A., Borisov, V. V., and Popova, A. P., “Methodolgoical approaches to studies of anthracosilicosis as a risk factor for morphological abnormalities,” Kompleks. Probl. Serdechno-Sosud. Zabol., No. 3, 23-27 (2015).
Kade, A. Kh., Zanin, S. A., Gubareva, E. A., Turovaya, A. Yu., Bogdanova, Yu. A., and Apsalyamova, S. O. “Physiological functions of the vascular endothelium,” Fundament. Issled., 11. No. 3, 611-617 (2011).
Silant’eva, T. A. and Krasnov, V. V., “Inraosseous blood supply of the pelvic girdle in dogs,” Nauchn. Vedom. BelGU, 18, No. 17, 111-120 (2014).
Ir’yanov, Yu. M., “A means of preparing bone specimens,” Patent application, USSR 1538184; No. 4129732/14; application date July 2, 1986; published January 23, 1990. Byul. No. 3, p. 234.
Silant’eva, T. A., Gorbach, E. N., Ir’yanov, Yu. M., Stupina, T. A., and Vorsegova, T. N., “A means of preparing biological tissues specimens for examination under the electron microscope,” Patent No. RUS 2397472. IPC: G01N1/28, G09B23/08. Application: 2008150910/12, December 22, 2008; Published August 20, 2010. Byul. No. 23.
Brückle, I., Thornton, J., Nichols, K., and Stricklerjaic, G., “Cyclododecane: Technical note on some uses in paper and objects,” JAIC, 38, No. 2, A4, 162-175 (1999).
Zav’yalova, O. V., Spivakovskii, Yu. M., Zakharova, N. B., Chernenkov, Yu. V., and Zlobina, O. V., “Angiogenesis and vascular endothelial growth factor, role in the pathology of the gastrointestinal tract,” Eksperim. Klin. Gastroenterol., 110, No. 10, 77-82 (2014).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Meditsinskaya Tekhnika, Vol. 53, No. 1, Jan.-Feb., 2019, pp. 13-16.
Rights and permissions
About this article
Cite this article
Ir’yanov, Y.M. Studies of Vessels in the Microcirculatory Bed of Regenerated Bone by Scanning Electron Microscopy. Biomed Eng 53, 18–21 (2019). https://doi.org/10.1007/s10527-019-09869-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10527-019-09869-1