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Suppression of phenylalanine ammonia-lyase activity elicited in date palm by Fusarium oxysporum f. sp. albedinis hyphal wall elicitor

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Biologia Plantarum

Abstract

The inoculation of the seedling roots of the resistant (Bousthami Noir) and susceptible (Jihel) date palm (Phoenix dactylifera) cultivars by Fusarium oxysporum f. sp. albedinis induced an increase in phenylalanine ammonia-lyase (PAL) activity. The response of the PAL activity in the resistant cultivar was faster and higher than in the susceptible one. However, the elicitation of the seedlings with the hyphal wall elicitor (HWE) of the pathogen induced identical PAL activity in both cultivars. In the resistant cultivar, the the PAL activity elicited with the HWE was not influenced by the addition of the fungal culture filtrate (FCF) whereas it was suppressed in the susceptible cultivar. This FCF suppressor effect was dose-dependent, not influenced by sodium periodate, whereas it was strongly reduced by the heat (121 °C for 45 min) and pronase E. These results show that differential induction of the defence mechanisms in both cultivars was not related to differences in the induction of the PAL activity, but to the suppression of its elicitation in the susceptible cultivar.

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Abbreviations

Foa:

Fusarium oxysporum f. sp. albedinis

FCF:

fungal culture filtrate

HWE:

hyphal wall elicitor

PAL:

phenylalanine ammonia-lyase

References

  • Amano, M., Toyoda, K., Ichinoise, Y., Yamada, T., Shiraishi, T.: Association between ion fluxes and defense responses in pea and cowpea tissues.-Plant Cell Physiol. 38: 698–706, 1997.

    PubMed  CAS  Google Scholar 

  • Ebel, J., Schmidt, W.E., Loyal, R.: Phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonialyase and chalchone synthase mRNAs and correlation with phytoalexin accumulation.-Arch. Biochem. Biophys. 232: 240–248, 1984.

    Article  PubMed  CAS  Google Scholar 

  • El Modafar, C., El Boustani, E.: Relationship between cell wall susceptibility to cellulases and pectinases of Fusarium oxysporum and susceptibility of date palm cultivars to the pathogen.-Biol. Plant. 43: 571–576, 2000.

    Article  Google Scholar 

  • El Modafar, C., El Boustani, E.: Cell wall-bound phenolic acid and lignin contents in date palm as related to its resistance to Fusarium oxysporum.-Biol. Plant. 44: 125–130, 2001.

    Article  Google Scholar 

  • El Modafar, C., El Boustani, E.: The role of phenolics in plant defense mechanisms.-In: Regnault-Roger, C, Philogène, B.J.R, Vincent, C. (ed.): Biopesticides of Plant Origin. Pp. 157–172. Intercept, Andover 2005.

    Google Scholar 

  • El Modafar, C., Tantaoui, A., El Boustani, E.: Time course accumulation and fungitoxicity of date palm phytoalexins towards Fusarium oxysporum f. sp. albedinis.-J. Phytopathol. 147: 477–484, 1999.

    Article  Google Scholar 

  • El Modafar, C., Tantaoui, A., El Boustani, E.: Effect of caffeoylshikimic acid of date palm roots on activity and production of Fusarium oxysporum f. sp. albedinis cell wall-degrading enzymes.-J. Phytopathol. 148: 101–108, 2000a.

    Article  Google Scholar 

  • El Modafar, C., Tantaoui, A., El Boustani, E.: Changes in cell wall-bound phenolic compounds and lignin in roots of date palm cultivars differing in susceptibility to Fusarium oxysporum f. sp. albedinis.-J. Phytopathol. 148: 405–411, 2000b.

    Article  Google Scholar 

  • El Modafar, C., Tantaoui, A., El Boustani, E.: Differential induction of phenylalanine ammonia-lyase activity in date palm roots in response to inoculation with Fusarium oxysporum f. sp. albedinis and to elicitation with fungal wall elicitor.-J. Plant Physiol. 158: 715–722, 2001.

    Article  Google Scholar 

  • Hahlbrock, K., Scheel, D.: Physiology and molecular biology of phenylpropanoid metabolism.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 347–369, 1989.

    Article  CAS  Google Scholar 

  • Hahn, M.G., Cheong, J.J., Alba, R., Enkerli, J., Côté, F.: Oligosaccharide elicitors: structure and recognition.-In: Fritig, B., Legrand M. (ed.): Mechanisms of Plant Defense Responses. Pp. 99–116. Kluwer Academic Publishers, Dordrecht 1993.

    Google Scholar 

  • Jones, D.H.: Phenylalanine ammonia-lyase: regulation of its induction, and its role in plant development.-Phytochemistry 23: 1349–1359, 1984.

    Article  CAS  Google Scholar 

  • Lu, H., Higgings, V.J.: Partial characterization of a nonproteinaceous suppressor of non-specific elicitors from Cladosporium fulvum (syn. Fulvia fulva).-Physiol. mol. Plant Pathol. 42: 427–439, 1993.

    Article  CAS  Google Scholar 

  • Nicholson, R.L., Hammerschmidt, R.: Phenolic compounds and their role in disease resistance.-Annu. Rev. Phytopathol. 30: 369–89, 1992.

    Article  CAS  Google Scholar 

  • Repka, V., Fischerová, I., Šilharová, K.: Methyl jasmonate is a potent elicitor of multiple defense responses in grapevine leaves and cell-suspension cultures.-Biol. Plant. 48: 273–283, 2004.

    Article  CAS  Google Scholar 

  • Sedra, M.H., Besri, M.: Evaluation de la résistance du palmier dattier au bayoud causé par Fusarium oxysporum f. sp. albedinis. Recherches d’une méthode de discrimination des vitroplants acclimatés en serre.-Agronomie 14: 467–472, 1994.

    Google Scholar 

  • Seki, H, Nagasugi, Y., Ichinose, Y., Shiraishi, T., Yamada, T.: Changes in vivo DNA-protein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules.-Plant Cell. Physiol. 40: 88–95, 1999.

    PubMed  CAS  Google Scholar 

  • Shiraishi, T., Toyoda, K., Yamada, T., Ichinose, Y., Kiba, A., Sugimoto, M.: Suppressors of defense-supprescins and plant receptor molecules.-In: Keen, N.T. (ed.): Delivery and Perception of Pathogen Signals in Plants. Pp. 112–121. APS Press, St. Paul 2001.

    Google Scholar 

  • Shiraishi, T., Yamada, T., Ichinose, Y., Kiba A., Toyoda, K., Kato, T., Murakami, Y., Seki, H.: Suppressor as a factor determining plant-pathogen specificity.-In: Stacey, G., Keen, N.T. (ed.): Plant-Microbe Interactions. Pp. 121–161. APS Press, St. Paul 1999.

    Google Scholar 

  • Shiraishi, T., Yamada, T., Oku, H., Yoshioka, H.: Suppressor production as a key factor for fungal pathogenesis.-In: Patil, S.S., Ouchi, S., Mills, D., Vance, D. (ed.): Molecular Strategies of Pathogens and Host Plants. Pp. 83–94. Springer-Verlag, New York 1991.

    Google Scholar 

  • Vidhyasekaran, P., Borromeo, E.S., Mew, T.W.: Helminthosporium oryzae toxin suppresses phenol metabolism in rice plants and aids pathogen colonization.-Physiol. mol. Plant Pathol. 41: 307–315, 1992.

    Article  CAS  Google Scholar 

  • Vurro, M., Ellis, B.E.: Effects of fungal toxins on induction of phenylalanine ammonia-lyase activity in elicited cultures of hybrid poplar.-Plant Sci. 126: 29–38, 1997.

    Article  CAS  Google Scholar 

  • Wada, M., Kato, H., Malik, K., Sriprasertsak, P., Ichinoise, Y., Shiraishi, T., Yamada, T.: A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase.-J. mol. Biol. 249: 513–519, 1995.

    Article  PubMed  CAS  Google Scholar 

  • Weisshaar, B., Jenkins, G.I.: Phenylpropanoid biosynthesis and its regulation.-Curr. Opin. Plant Biol. 1: 251–257, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, T., Shiraishi, T., Ichinose, Y., Kato, H., Seki, H., Murakami, Y.: Regulation of genes for phenylpropanoid synthesis in pea by elicitor and suppressor.-In: Mills, D., Kunoch, H., Keen, N.T., Mayama, S. (ed.): Molecular Aspects of Pathogenecity and Resistance. Requirement for Signal Transduction. Pp. 151–162. Amer. Phytopathol. Soc., St. Paul 1996.

    Google Scholar 

  • Yamada, T., Sriprasertsak, P., Kato, H., Hashimoto, T., Shimizu, H., Shiraishi, T.: Functional analysis of promoters of phenylalanine ammonia-lyase genes in pea.-Plant Cell Physiol. 35: 917–926, 1994.

    PubMed  CAS  Google Scholar 

  • Ziouti, A., El Modafar, C., El Mandili, A., El Boustani, E., Macheix, J.J.: Identification des acides caféoylshikimiques des racines du palmier dattier, principaux composés fongitoxiques vis-à-vis du Fusarium oxysporum f. sp. albedinis.-J. Phytopathol. 144: 197–202, 1996.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biotechnology and Molecular Phytopathology, Faculty of Sciences and Technology of Gueliz, P.O. Box 549, 40 000, Marrakech, Morocco

    C. El Modafar, B. Rahioui, A. El Meziane & Z. El Alaoui-Talibi

  2. Laboratory of Nutritional and Pathological Biochemistry, Faculty of Sciences of Semlalia, 40 000, Marrakech, Morocco

    E. El Boustani

Authors
  1. C. El Modafar
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  2. E. El Boustani
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  3. B. Rahioui
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  4. A. El Meziane
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  5. Z. El Alaoui-Talibi
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Correspondence to C. El Modafar.

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El Modafar, C., El Boustani, E., Rahioui, B. et al. Suppression of phenylalanine ammonia-lyase activity elicited in date palm by Fusarium oxysporum f. sp. albedinis hyphal wall elicitor. Biol Plant 50, 697–700 (2006). https://doi.org/10.1007/s10535-006-0109-y

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  • DOI: https://doi.org/10.1007/s10535-006-0109-y

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