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Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress

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Biologia Plantarum

Abstract

In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbic acid

CAT:

catalase

CK:

control

F0 :

initial fluorescence

Fv/Fm :

variable to maximum fluorescence ratio (maximum photochemical efficiency of PS 2)

MDA:

malondialdehyde

O2 ·− :

superoxide radical

PBS:

phosphate-buffered saline

PN :

net photosynthetic rate

PS 2:

photosystem 2

REL:

relative electrolyte leakage

ROS:

reactive oxygen species

SDS:

sodium dodecyl sulfate

SOD:

superoxide dismutase

SSC:

standard saline citrate

tAPX:

thylakoid-bound ascorbate peroxidase

TBA:

Tris-buffered acetate

TCA:

trichloroacetic acid

tAPX :

tomato thylakoid-bound ascorbate peroxidase gene

WT:

wild type plant

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Acknowledgements

This research was supported by the State Key Basic Research and Development Plan of China (2009CB118500), the Natural Science Foundation of China (30871458) and Program for Changjiang Scholars and Innovative Research Team in University (Grant IRT0635).

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Authors and Affiliations

  1. College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, P.R. China

    W. -H. Sun, M. Duan, F. Li, D. -F. Shu, S. Yang & Q. -W. Meng

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  1. W. -H. Sun
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  2. M. Duan
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  3. F. Li
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  4. D. -F. Shu
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  5. S. Yang
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  6. Q. -W. Meng
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Correspondence to Q. -W. Meng.

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Sun, W.H., Duan, M., Li, F. et al. Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress. Biol Plant 54, 614–620 (2010). https://doi.org/10.1007/s10535-010-0111-2

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  • DOI: https://doi.org/10.1007/s10535-010-0111-2

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