Abstract
In this study we investigated the effects of boric acid and sodium tetraborate on an acute leukemia cell line and healthy human lymphocytes. We evaluated the effects of boric acid and sodium tetraborate on the HL-60 cell line and healthy human lymphocytes by using the methods of MTT, Neutral Red, AO (flow cytometry) and transmission electron microscope. We found that there were dead cells at a concentration of 500 µM boric acid and sodium tetraborate (50 % and 40 %, respectively). An apoptotic effect was found at a concentration of 1,000 µM concentration in normal lymphocytes and HL-60 (acute leukemia cells) cells (2.5 % and 8.8 % respectively). We observed that boric acid at a concentration of 500 µM caused double nucleus and micronucleus formation in both HL-60 cells and lymphocytes. An expansion in mitochondrial dimension and deformation in cristas also appeared. Our findings suggest that boric acid is more effective than sodium tetraborate on the HL-60, and boric acid in particular showed a cytotoxic effect on HL-60 in comparison to healthy lymphocytes and it also affected the mitochondrial pathway.
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Acknowledgments
Flow cytometry analyses for the present work were carried out at the Department of Hematology, School of Medicine, Eskişehir Osmangazi University, Eskişehir. The authors gratefully thank Dr. Ayhan Bilir, Department of Histology and Embryology, Faculty of Medicine, Istanbul University, Istanbul, Turkey. The authors declare that there are no conflicts of interest.
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Canturk, Z., Tunali, Y., Korkmaz, S. et al. Cytotoxic and apoptotic effects of boron compounds on leukemia cell line. Cytotechnology 68, 87–93 (2016). https://doi.org/10.1007/s10616-014-9755-7
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DOI: https://doi.org/10.1007/s10616-014-9755-7