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Hydroxysafflor yellow A (HYSA) inhibited the proliferation and differentiation of 3T3-L1 preadipocytes

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Abstract

Hydroxysafflor yellow A (HSYA), a main component of safflor yellow, has been demonstrated to prevent steroid-induced avascular necrosis of femoral head by inhibiting primary bone marrow-derived mesenchymal stromal cells adipogenic differentiation induced by steroid. In this study, we investigate the effect of HSYA on the proliferation and adipogenesis of mouse 3T3-L1 preadipocytes. The effects of HSYA on proliferation and differentiation of 3T3-L1 cells and its possible mechanism were studied by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide spectrophotometry, Oil Red O staining, intracellular triglyceride assays, real-time quantitative RT-PCR, transient transfection and dual luciferase reporter gene methods. HSYA inhibited the proliferation of 3T3-L1 preadipocytes and cell viability greatly decreased in a dose and time dependent manner. HSYA (1 mg/l) notably reduced the amount of intracellular lipid and triglyceride content in adipocytes by 21.3 % (2.13 ± 0.36 vs 2.71 ± 0.40, P < 0.01) and 22.6 % (1.33 ± 0.07 vs 1.72 ± 0.07, P < 0.01) on days 8 following the differentiation, respectively. HSYA (1 mg/l) significantly increased hormone-sensitive lipase (HSL) mRNA expression and promoter activities by 2.4- and 1.55-fold, respectively (P < 0.01), in differentiated 3T3-L1 adipocytes. HSYA inhibits the proliferation and adipogenesis of 3T3-L1 preadipocytes. The inhibitory action of HYSA on adipogenesis may be due to the promotion of lipolytic-specific enzyme HSL expression by increasing HSL promoter activity.

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Abbreviations

HSYA:

Hydroxysafflor yellow A

HSL:

Hormone-sensitive lipase

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Acknowledgments

The study was supported by grants from the National Natural Science Foundation of China (Nos. 30600836, 81471024 for Zhu HJ, Nos. 30540036, 30771026, 81370898 for Gong FY), the Beijing Natural Science Foundation (No. 7082079 for Gong FY), the National Key Program of Clinical Science (WBYZ2011-873 for Gong FY and Zhu HJ) and PUMCH (2013-020 for Gong FY).

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Authors and Affiliations

  1. Department of Endocrinology, Key Laboratory of Endocrinology of Ministry of Health, The Translational Medicine Center of PUMCH, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 1# Shuaifuyuan, Wangfujing, Beijing, 100730, China

    Hui-juan Zhu, Lin-jie Wang, Xiang-qing Wang, Hui Pan, Nai-shi Li, Hong-bo Yang & Feng-ying Gong

  2. Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, China-Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, 100029, China

    Ming Jin & Bao-xia Zang

Authors
  1. Hui-juan Zhu
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  2. Lin-jie Wang
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  4. Hui Pan
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  5. Nai-shi Li
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  7. Ming Jin
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  9. Feng-ying Gong
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Correspondence to Feng-ying Gong.

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Zhu, Hj., Wang, Lj., Wang, Xq. et al. Hydroxysafflor yellow A (HYSA) inhibited the proliferation and differentiation of 3T3-L1 preadipocytes. Cytotechnology 67, 885–892 (2015). https://doi.org/10.1007/s10616-014-9783-3

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  • DOI: https://doi.org/10.1007/s10616-014-9783-3

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