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A standardised method for the quantitative analysis of resistance to grapevine powdery mildew

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Abstract

Powdery mildew caused by Erysiphe necator is one of the most important diseases affecting grapevine (Vitis vinifera, L.). Control of this pathogen is based on the use of fungicides, which cause environmental damage and increase production costs. A cost-effective and environmentally friendly alternative to control the disease relies on using resistant varieties. While most V. vinifera cultivars are susceptible to powdery mildew, several species belonging to the Vitaceae have been described as resistant. Several loci for resistance to grapevine powdery mildew have been identified through genetic analysis of segregating populations derived from different resistance sources. Identifying quantitative trait loci (QTL) with minor effects on the resistance may prove valuable in a strategy of pyramiding, which aims at increasing the durability of the resistance. However, current methods for evaluation of resistance either do not take into account quantitative variations, or, if they do, are not adapted to large sample sets. Here we develop a method for the analysis of quantitative resistance to grapevine powdery mildew in large populations. We devised a semi-quantitative resistance scale and confirmed the usefulness of a cell counter to quantify sporulation. We compared three inoculation methods and identified dry inoculation using a settling tower as the one giving the best infection. Finally, we confirmed the value of the method by applying it to a set of plants segregating for resistance to E. necator. Using the method described here for the quantitative analysis of the resistance to powdery mildew will prove valuable for breeding for durable resistance.

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Acknowledgements

We thank Marie-Annick Dorne for assistance with powdery mildew inoculum, Pascale Coste for assistance with plant material and Sophie Blanc for helpful discussions on the resistance indexes. The comments and suggestions from an anonymous reviewer, which contributed to improve the quality of this paper, are acknowledged. Research funded by the French “Agence Nationale de la Recherche”, grant number ANR-08-GENM-007.

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Author notes

  1. Anne-Sophie Miclot

    Present address: UMR Génétique et Horticulture (GenHort), IFR149 QUASAV, Centre INRA Angers-Nantes, BP 60057, 49071, Beaucouzé, France

Authors and Affiliations

  1. INRA, UMR 1131 Santé de la Vigne et Qualité du Vin, F-68000, Colmar, France

    Anne-Sophie Miclot, Sabine Wiedemann-Merdinoglu, Eric Duchêne, Didier Merdinoglu & Pere Mestre

  2. Université de Strasbourg, UMR 1131 Santé de la Vigne et Qualité du Vin, F-68000, Colmar, France

    Anne-Sophie Miclot, Sabine Wiedemann-Merdinoglu, Eric Duchêne, Didier Merdinoglu & Pere Mestre

  3. UMR 1131 Santé de la Vigne et Qualité du Vin, 28 rue de Herrlisheim, BP 20507, 68021, Colmar cedex, France

    Pere Mestre

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  1. Anne-Sophie Miclot
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Correspondence to Pere Mestre.

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Miclot, AS., Wiedemann-Merdinoglu, S., Duchêne, E. et al. A standardised method for the quantitative analysis of resistance to grapevine powdery mildew. Eur J Plant Pathol 133, 483–495 (2012). https://doi.org/10.1007/s10658-011-9922-z

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