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Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts

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Abstract

A rapid, simple, accurate, and sensitive visible spectrophotometric method for the determination of trace amounts of hydrogen peroxide in acidic buffer medium is reported. The proposed method is based on the oxidative coupling of Ampyrone with dibenzazepin hydrochloride by hydrogen peroxide in the buffer medium of pH 4.0 which is catalyzed by ferrous iron. The blue-colored product formed with maximum absorption at 620 nm was found to be stable for 2 h. Beer’s law is obeyed for hydrogen peroxide concentration in the range of 0.03–0.42 μg ml−1. The optimum reaction conditions and other important optical parameters are reported. The molar absorptive and Sandell’s sensitivity are found to be 5.89 × 104 mol−1 cm−1 and 0.57 g/cm2, respectively. The interference due to diverse ions and complexing agents was studied. The method is successfully applied to the determination of hydrogen peroxide in green plants satisfactorily.

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Acknowledgment

One of the authors (JSP) would like to thank the University of Mysore for the laboratory facilities.

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Authors and Affiliations

  1. Department of Studies in Chemistry, University of Mysore, Mansagangotri, Mysore, 570 006, India

    P. Nagaraja

  2. Department of Chemistry, Sri Bhagawan Mahaveer Jain College of Engineering, Jakkasandra, Karnataka, 562 112, India

    J. S. Prakash & S. C. Asha

  3. Department of Chemistry, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Bangalore Campus, Bangalore, Karnataka, 560035, India

    B. L. Bhaskara & S. Anil Kumar

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  1. P. Nagaraja
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  2. J. S. Prakash
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  3. S. C. Asha
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  4. B. L. Bhaskara
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  5. S. Anil Kumar
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Correspondence to B. L. Bhaskara.

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Nagaraja, P., Prakash, J.S., Asha, S.C. et al. Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts. Environ Monit Assess 184, 5983–5988 (2012). https://doi.org/10.1007/s10661-011-2395-x

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  • DOI: https://doi.org/10.1007/s10661-011-2395-x

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