Abstract
The aim of this study was to assess the effect of the mutant genes B, og c, hp, and t on the fruit color of tomato genotypes. Two tomato lines (TOM-498 and TOM-499) with BB genotype composition (high β-carotene homozygous) were used in hybrid combinations with 7 tomato lines with different genotype compositions in loci og c, hp, or t—Floradade, TOM-596, TOM-544, NC-8276, NC-2Y, Florida 7775, and Florida 7781. A randomized complete block design with 24 treatments and four replications was used. Both external and internal fruit color were assessed with a Minolta CR-400 colorimeter in the CIE L*, a* and b* mode. Hue and chroma readings were taken at four different points (epidermis, pericarp, placenta, and columella) of the fruit. For high β-carotene heterozygous hybrids B + /B, hue angle indicated an orange color (since these hybrids did not come to equal the genotypes of normal coloring, red), even when genes promoting lycopene synthesis (og c, hp) were deployed as heterozygous genotypes. Fruit of high β-carotene heterozygous hybrids, without t, og c, or hp, had chroma values similar to fruit of homozygous high β-carotene lines. The use of the og c allele in heterozygosity led to higher chroma values in high β-carotene heterozygous genotypes in relation to the values found in high β-carotene heterozygous genotypes not bearing the og c gene. The og c homozygous lines had hue values that did not differ from those in normal genotypes in the epidermis and pericarp, but showed a significant shift towards red in the placenta and in the columella.
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Acknowledgments
The authors thank the following Brazilian institutions that provide support for this research Project: FAPEMIG-Fundação de Amparo à Pesquisa do Estado de Minas Gerais, CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico, FINEP—Financiadora de Estudos e Projetos/MCT-Ministério de Ciência e Tecnologia/FNDCT—Fundo Nacional de Desenvolvimento Científico e Tecnológico, CAPES-Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/MEC-Ministério da Educação Brasileiro, UFLA-Universidade Federal de Lavras, HortiAgro Sementes S.A., FUNDECC-Fundação para o Desenvolvimento Científico e Tecnológico, and FAEPE-Fundação de Apoio ao Ensino, Pesquisa e Extensão.
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Andrade, T.M., Maluf, W.R., de Oliveira, C.M. et al. Interaction of the mutant genes B, og c , hp and t in the coloring of tomato fruit. Euphytica 205, 773–783 (2015). https://doi.org/10.1007/s10681-015-1405-4
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DOI: https://doi.org/10.1007/s10681-015-1405-4