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Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.)

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Abstract

The identity and expression of S-RNase genotypes in the self-compatible (SC) apricot cultivar ‘Katy’ and the self-incompatible (SI) cultivar ‘Xinshiji’ were examined. We used allele specific polymerase chain reaction (AS-PCR) and designated the alleles in ‘Katy’ and ‘Xinshiji’ as S8Sc and S9S10, respectively. The S-RNase gene was expressed in style at the balloon stage in both genotypes. Using real-time fluorescence quantification RT-PCR technology (FQRT-PCR), spatio-temporal expression patterns of S-RNase gene between ‘Katy’ and ‘Xinshiji’ were compared. The results revealed that the expression of the S-RNase gene in ‘Katy’ and ‘Xinshiji’ were different. The transcript abundance was distinctly diverse at the key stage (i.e., at 24 h after self-pollination) in both genotypes, and was greater in ‘Xinshiji’ (SI) than ‘Katy’ (SC). In addition, the abundance of the S-RNase transcript was higher in upper-half of style than in the lower-half of style or in the ovary. In the SI cultivar ‘Xinshiji’, the expression of S-RNase reminded a relatively high level after cross-pollination, but it dropped continuously after self-pollination and un-pollination.

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Abbreviations

ACP:

After cross-pollination

AS-PCR:

Allele specific polymerase chain reaction

ASP:

After self-pollination

BA:

Before anthesis

BS:

At balloon stage

FQRT-PCR:

Fluorescence quantification RT-PCR technology

RT-PCR:

Reverse transcript-PCR

SI:

Self-incompatible

SC:

Self-compatible

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Authors and Affiliations

  1. Horticultural Science and Engineering College, Shandong Agricultural University, Daizong Street 61, 271018, Taian, Shandong, China

    Jianrong Feng, Xuesen Chen, Yan Wu, Wen Liu, Qing Liang & Lijie Zhang

  2. Horticultural Department, Shihezi University, Shihezi, Xinjiang, 832003, China

    Jianrong Feng

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  1. Jianrong Feng
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  2. Xuesen Chen
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  3. Yan Wu
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  4. Wen Liu
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  5. Qing Liang
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  6. Lijie Zhang
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Correspondence to Xuesen Chen.

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Feng, J., Chen, X., Wu, Y. et al. Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.). Mol Biol Rep 33, 215–221 (2006). https://doi.org/10.1007/s11033-006-0011-x

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  • DOI: https://doi.org/10.1007/s11033-006-0011-x

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