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Heterologous synthesis and assembly of functional LHII antenna complexes from Rhodovulum sulfidophilum in Rhodobacter sphaeroides mutant

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Abstract

The light harvesting complexes, including LHII and LHI, are the important components of photosynthetic apparatus. Rhodovulum (Rdv.) sulfidophilum and Rhodobacter (R.) sphaeroides belong to two genera of photosynthetic bacteria, and they are very different in some physiological characteristics and light harvesting complexes structure. The LHII structural genes (pucBsAs) from Rdv. sulfidophilum and the LHI structural genes (pufBA) from R. sphaeroides were amplified, and cloned into an expression vector controlled by puc promoter from R. sphaeroides, which was then introduced into LHI and LHII-minus R. sphaeroides mutants; the transconjugant strains synthesized heterologous LHII and native LHI complexes, which played normal roles in R. sphaeroides. The Rdv. sulfidophilum LHII complex from pucBsAs had near-infrared absorption bands at ~801–853 nm in R. sphaeroides, and was able to transfer energy efficiently to the native LHI complex. The results show that the pucBsAs genes from Rdv. sulfidophilum could be expressed in R. sphaeroides, and the functional foreign LHII and native LHI were assembled into the membrane of R. sphaeroides.

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Abbreviations

LHII:

Light-harvesting II

LHI:

Light-harvesting I

RC:

Reaction center

LB:

Luria–Bertani medium

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-PCR

PMSF:

Phenyl methyl sulfonyl fluoride

EDTA:

Ethylene diamine tetraacetic acid

LDAO:

Lauryl N, N-dimethylamine-N-oxide

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Acknowledgments

This work was supported by the Program “863” of the Ministry of Science and Technology of the People’s Republic of China (NO:2006AA02Z138) and Natural Science Foundation of the Chongqing Science and Technology Commission (NO: CSTC,2006BA5006). We appreciate C. Neil Hunter for offering the strain DD13, and also acknowledge the intellectual support of my colleagues in our laboratory.

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Authors and Affiliations

  1. College of Bioengineering, Chongqing University, Shazhengjie 174, Shapingba, Chongqing, China

    Wanneng Wang, Zongli Hu, Xuqing Chen, Zhiping Zhao, Jinzhe Li & Guoping Chen

  2. Department of Bioengineering, Chongqing Institute of Technology, Xingshenglu 4, Yangjiaping, Chongqing, China

    Wanneng Wang

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  1. Wanneng Wang
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Correspondence to Guoping Chen.

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Wang, W., Hu, Z., Chen, X. et al. Heterologous synthesis and assembly of functional LHII antenna complexes from Rhodovulum sulfidophilum in Rhodobacter sphaeroides mutant. Mol Biol Rep 36, 1695–1702 (2009). https://doi.org/10.1007/s11033-008-9370-9

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