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Low-temperature time-resolved spectroscopic study of the major light-harvesting complex of Amphidinium carterae

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Abstract

The major light-harvesting complex of Amphidinium (A.) carterae, chlorophyll-a–chlorophyll-c 2–peridinin–protein complex (acpPC), was studied using ultrafast pump-probe spectroscopy at low temperature (60 K). An efficient peridinin–chlorophyll-a energy transfer was observed. The stimulated emission signal monitored in the near-infrared spectral region was stronger when redder part of peridinin pool was excited, indicating that these peridinins have the S1/ICT (intramolecular charge-transfer) state with significant charge-transfer character. This may lead to enhanced energy transfer efficiency from “red” peridinins to chlorophyll-a. Contrary to the water-soluble antenna of A. carterae, peridinin–chlorophyll-a protein, the energy transfer rates in acpPC were slower under low-temperature conditions. This fact underscores the influence of the protein environment on the excited-state dynamics of pigments and/or the specificity of organization of the two pigment–protein complexes.

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Acknowledgments

The authors thank Miriam Enriquez for help with measuring the absorption spectra at 77 K. The work in Czech Republic was supported by the Czech Science Foundation (P501/12/G055 and P205/11/1164), and project KONTAKT ME09037 from the Ministry of Education of the Czech Republic. Work in the laboratory of HAF was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation.

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Authors and Affiliations

  1. Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Václav Šlouf, Marcel Fuciman & Tomáš Polívka

  2. AG Proteincrystallography, Department of Biophysics, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, 44780, Bochum, Germany

    Silke Johanning & Eckhard Hofmann

  3. Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT, 06269-3060, USA

    Marcel Fuciman & Harry A. Frank

  4. Biological Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Tomáš Polívka

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  1. Václav Šlouf
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  2. Marcel Fuciman
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  3. Silke Johanning
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  4. Eckhard Hofmann
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  5. Harry A. Frank
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  6. Tomáš Polívka
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Correspondence to Tomáš Polívka.

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Šlouf, V., Fuciman, M., Johanning, S. et al. Low-temperature time-resolved spectroscopic study of the major light-harvesting complex of Amphidinium carterae . Photosynth Res 117, 257–265 (2013). https://doi.org/10.1007/s11120-013-9900-8

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  • DOI: https://doi.org/10.1007/s11120-013-9900-8

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