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Prognostic significance of combining high mobility group Box-1 and OV-6 expression in hepatocellular carcinoma

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Abstract

The inflammatory environment and existence of cancer stem cells are critical for progression and intrahepatic recurrence of hepatocellular carcinoma (HCC) after curative resections. Here, we investigated the prognostic significance of combining high mobility group box 1 (HMGB1) expression and hepatic progenitor marker OV6 in hepatocellular carcinoma. Expression of HMGB1 and OV6 was evaluated using immunohistochemistry profiling in tissue microarrays containing samples from 208 HCC patients. Invasive clinical or pathological factors were found in patients with high expression of HMGB1 or OV6. Higher HMGB1 was associated with poorer clinical outcomes, and independently related to elevated 5-year recurrence incidence (85.5% vs. 62.4%, P<0.001). We also found that more OV6 positive staining was correlated with poor prognosis of HCC patients (P<0.001). Notably, expression of HMGB1 was positively correlated with OV6 in density (R2=0.032, P<0.001) and reversely related to HCC outcomes. Abnormal expression of HMGB1 in combination with positive staining of OV6 displayed poorer prognostic performance than single biomarker alone (area under curve (AUC) survival=0.696). Therefore, HMGB1 and OV6 positive staining are promising prognostic parameters for HCC, and we propose that HMGB1 and OV6 may cooperate with each other and predict poor prognosis of HCC.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81370061, 81521091, 81572896, 81370137, 81722034) and the National Science and Technology Key Projects (2017ZX10203205, 2017ZX10302202).

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Authors and Affiliations

  1. National Center for Liver Cancer, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China

    Jihui Zhu, Han Yu, Shuzhen Chen, Zihui Dong, Yan Ling, Wen Yang, Liang Tang, Hongyang Wang & Wen Wen

  2. International Cooperation Laboratory on Signal Transduction of Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200433, China

    Jihui Zhu, Han Yu, Shuzhen Chen, Zihui Dong, Yan Ling, Wen Yang, Liang Tang, Hongyang Wang & Wen Wen

  3. Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200433, China

    Pinghua Yang, Shilei Bai & Feng Shen

  4. Department of Respiratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Hao Tang

  5. Ministry of Education Key Laboratory on Signaling Regulation and Targeting Therapy of Liver Cancer, Shanghai, 200438, China

    Hongyang Wang & Wen Wen

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  1. Jihui Zhu
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Zhu, J., Yu, H., Chen, S. et al. Prognostic significance of combining high mobility group Box-1 and OV-6 expression in hepatocellular carcinoma. Sci. China Life Sci. 61, 912–923 (2018). https://doi.org/10.1007/s11427-017-9188-x

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