Abstract
The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae XJPD-Li under different aeration strategies were investigated. In batch fermentation, the results showed that the final concentration of 1,3-PD and yield on glycerol were 13.44 g/l and 0.73 mol/mol under the anaerobic condition (N2, 0.4 vvm), 11.55 g/l and 0.62 mol/mol without aeration, and 8.73 g/l and 0.47 mol/mol under the aerobic condition (air, 0.4 vvm), respectively. Under the aerobic condition, the yield of 1,3-PD on glycerol was the lowest, while the biomass (optical density at 650 nm) was the highest among these three conditions. In the fed-batch culture, the final concentration and the yield of 1,3-PD was 60.82 g/l and 0.61 mol/mol under the anaerobic condition (N2, 0.4 vvm), 56.43 g/l and 0.53 mol/mol without aeration, and 65.26 g/l and 0.56 mol/mol under the aerobic condition. All these three conditions had good productivities of 1,3-PD, which were 3.35 g/l·h under the anaerobic condition (N2, 0.4 vvm), 3.13 g/l·h without aeration, and 3.16 g/l·h under the aerobic condition within the initial 12 h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biebl, H., Menzel, K., Zeng, A. P., & Deckwer, W. D. (1999). Microbial production of 1, 3-propanediol. Applied Microbiology and Biotechnology, 52, 289–297.
Zhao, Y. N., Chen, G., & Yao, S. J. (2006). Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae. Biochemical Engineering Journal, 32, 93–99.
Nakamura, C. E., & Whited, G. M. (2003). Metabolic engineering for the microbial production of 1,3-propanediol. Current Opinion in Biotechnology, 14, 454–459.
Zheng, P., Wereath, K., Sun, J. B., Heuvel, J., & Zeng, A. P. (2006). Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae. Process Biochemistry, 41, 2160–2169.
Tobimatsu, T., Azuma, M., Matsubara, H., Takatori, H., Niida, T., Nishimoto, K., et al. (1996). Cloning, sequence, and high level expression of the genes encoding adenosylcobalamin-dependent glycerol dehydrase of Klebsiella pneumoniae. Journal of Biological Chemistry, 271, 22352–22357.
Rolf, D., Bobik, T. A., & Gottschalk, G. (1999). Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes. FEMS Microbiology Reviews, 22, 553–566.
Chen, X., Zhang, D. J., Qi, W. T., Gao, S. J., Xiu, Z. L., & Xu, P. (2003). Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions. Applied Microbiology and Biotechnology, 63, 143–146.
Cheng, K. K., Liu, D. H., Sun, Y., & Liu, W. B. (2004). 1, 3-Propanediol production by Klebsiella pneumoniae under different aeration strategies. Biotechnology Letters, 26, 911–915.
Lin, R. H., Liu, H. J., Sun, Y., Cheng, K. K., & Liu, D. H. (2006). Effect of oxygen on the metabolism of Klebsiella pneumoniae in 1,3-propanediol fermentation(Chinese). The Chinese Journal of Process Engineering, 6(1), 96–99.
Zhang, G. L., Ma, B. B., Xu, X. L., Li, C., & Wang, L. W. (2007). Fast conversion of glycerol to 1,3-propanediol by a new strain of Klebsiella pneumoniae. Biochemical Engineering Journal, 37, 256–260.
Bradford, M. M. (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye building. Analytical Biochemistry, 72, 248–254.
Toraya, T., Kuno, S., & Fukui, S. (1980). Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae. Journal of Bacteriology, 141, 1439–1442.
Zeng, A. P., Biebl, H., & Schlieker, H. (1993). Pathway analysis of glycerol fermentation by K. pneumoniae: regulation of reducing equivalent balance and product formation. Enzyme and Microbial Technology, 15, 770–779.
Menzel, K., Ahrens, K., Zeng, A. P., & Deckwer, W. D. (1998). Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: IV. Enzymes and fluxes of pyruvate metabolism. Biotechnology and Bioengineering, 60, 617–622.
Hartlep, M., Hussmann, W., Prayitno, N., Meynial-Salles, I., & Zeng, A. P. (2002). Study of two-stage processes for the microbial production of 1,3-propanediol from glucose. Applied Microbiology and Biotechnology, 60, 60–66.
Menzel, K., Zeng, A. P., & Deckwer, W. D. (1997). High concentration and productivity of 1,3-propanediol from continuous fermentation of glycerol by Klebsiella pneumoniae. Enzyme and Microbial Technology, 20, 82–86.
Zhang, Y. P., Du, C. Y., Rao, Z., & Cao, Z. A. (2005). Regulation of vitamin C and vitamin E on the biosynthesis of 1,3-propanediol by Klebsiella pneumoniae. The Chinese Journal of Process Engineering, 5, 197–200.
Zhang, Y. P., Li, Y., Du, C. Y., Liu, M., & Cao, Z. A. (2006a). Inactivation of aldehyde dehydrogenase: A key factor for engineering 1,3-propanediol production by Klebsiella pneumoniae. Metabolic Engineering, 8, 578–586.
Zhang, Y. P., Liu, M., Du, C. Y., Shen, J. Y., & Cao, Z. A. (2006b). Effect of by-products on cell growth and biosynthesis of 1,3-propanediol by Klebsiella pneumoniae. The Chinese Journal of Process Engineering, 6(5), 804–808 (in Chinese).
Acknowledgments
This work was financially supported by National Natural Science Foundation of China (2046002, 20776017), Natural Science Foundation of Beijing (5072028), Program for New Century Excellent Talents in University (NCET-04-0989), and Fok Ying Tung Education Foundation (101071).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ma, B.B., Xu, X.L., Zhang, G.L. et al. Microbial Production of 1,3-Propanediol by Klebsiella pneumoniae XJPD-Li under Different Aeration Strategies. Appl Biochem Biotechnol 152, 127–134 (2009). https://doi.org/10.1007/s12010-008-8220-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8220-5