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New Potential Biocatalysts by Laccase Immobilization in PVA Cryogel Type Carrier

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Abstract

Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme.

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Acknowledgements

This work was supported by the Romanian Academy (grant 1R-2008) and by the Russian Foundation for Basic Research (Project # 07-03-91682_RA_a).

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Authors and Affiliations

  1. Aurel Vlaicu University, Elena Dragoi str. 2, 310330, Arad, Romania

    Michaela Dina Stanescu, Magdalena Fogorasi & Simona Gavrilas

  2. A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov str. 28, 119991, Moscow, Russian Federation

    Boris L. Shaskolskiy & Vladimir I. Lozinsky

Authors
  1. Michaela Dina Stanescu
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  2. Magdalena Fogorasi
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  3. Boris L. Shaskolskiy
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  4. Simona Gavrilas
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  5. Vladimir I. Lozinsky
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Correspondence to Michaela Dina Stanescu.

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Stanescu, M.D., Fogorasi, M., Shaskolskiy, B.L. et al. New Potential Biocatalysts by Laccase Immobilization in PVA Cryogel Type Carrier. Appl Biochem Biotechnol 160, 1947–1954 (2010). https://doi.org/10.1007/s12010-009-8755-0

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  • DOI: https://doi.org/10.1007/s12010-009-8755-0

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