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The Determination of Fetal RhD Status from Maternal Blood in Serbia

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Abstract

The aim of this research was to demonstrate: The specificity, sensitivity, the positive and negative prediction value of the PCR method in the detection of the fetal RhD status from maternal blood in various weeks of gestation. Which was the earliest gestational week, when is possible to get a reliable result? How did the presence of RhD antibodies in sensitized mothers affect the results? This was a prospective clinical study within a multidisciplinary project approved by the Ministry of Science of the Republic of Serbia, 2011. The study was approved by the Ethics Committee of the Medical Faculty in Belgrade. Ninety six RhD negative patients had participated in the study, with written consent. The sensitivity of the determination of the RhD status of the fetus from the mother’s blood after the 7th GW in our sample was 98.6%, with a specificity of 92%. The positive predictive value of the test was 97% and the negative predictive value was 95.6%. False positive results were detected in 2.6% of cases and 4.5% of cases were false negative. This is a reliable test which should be used in monitoring of pregnancies of RhD negative mothers.

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Authors and Affiliations

  1. Clinic for Obstetrics and Gynecology, Clinical Center of Serbia, Medical School University Belgrade, Majke Jevrosime 8, 11000, Belgrade, Serbia

    S. Plešinac, D. Plećaš & I. Babović

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  1. S. Plešinac
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  2. D. Plećaš
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  3. I. Babović
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Correspondence to S. Plešinac.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Plešinac, S., Plećaš, D. & Babović, I. The Determination of Fetal RhD Status from Maternal Blood in Serbia. Indian J Hematol Blood Transfus 34, 486–490 (2018). https://doi.org/10.1007/s12288-018-0919-z

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  • DOI: https://doi.org/10.1007/s12288-018-0919-z

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