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Investigation of Lactic Acid Bacteria Isolated from Giant Panda Feces for Potential Probiotics In Vitro

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Abstract

The present study aimed to isolate an optimal lactic acid bacterial strain from the feces of healthy giant pandas. The strain exhibited good stability at low pH and high bile salt concentrations, activity against pathogens relevant to pandas, and antibiotic susceptibility. In the current study, 25 isolates were obtained from de Man, Rogosa, and Sharpe agar. Two (E21 and G83) and eight (E1, E2, E16, E18, E21, E69, E70, and G83) isolates demonstrated good performance at pH 2.0 and bile 2% (w/v), respectively. Three isolates (G83, G88, and G90) possessed better antimicrobial effect on enterotoxigenic Escherichia coli CVCC196 (ETEC) than the rest. One isolate (G83) strongly affected Salmonella, whereas three (G83, G87, and G88) exhibited inhibitory activity against Staphylococcus aureus. All isolates were multi-drug resistant. These isolates were identified as Lactobacillus (5 isolates) and Enterococcus (20 isolates) by 16S rRNA sequencing. Virulence genes were detected in Enterococcus isolates. Isolate G83 was identified as Lactobacillus plantarum and was considered as the best probiotic candidate among all of the experimental isolates. This study provided necessary and important theoretical guidance for further experiments on G83 in vivo.

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Funding

The present study was supported by the National Natural Science Foundation of China (31672318) and the Funded Project of Chengdu Giant Panda Breeding Research Foundation (CPF2014-15, CPF2015-06).

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    Authors and Affiliations

    1. Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China

      Qian Liu, Xueqin Ni, Zhirong Peng, Meiling Xie, Yicen Lin, Yi Zhou & Dong Zeng

    2. Chengdu Wildlife Institute, Chengdu Zoo, Chengdu, 610081, China

      Qiang Wang & Lili Niu

    3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China

      Hao Sun, Kangcheng Pan, Bo Jing & Dong Zeng

    Author notes

    1. Xueqin Ni, Qiang Wang and Zhirong Peng are joint first authors.

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      1. Qian Liu
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      Correspondence to Dong Zeng.

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      Liu, Q., Ni, X., Wang, Q. et al. Investigation of Lactic Acid Bacteria Isolated from Giant Panda Feces for Potential Probiotics In Vitro. Probiotics & Antimicro. Prot. 11, 85–91 (2019). https://doi.org/10.1007/s12602-017-9381-8

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